FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888149

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888149
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences266936
Sequences flagged as poor quality0
Sequence length301
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT90063.3738424191566514No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA62832.3537477148080437No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA62372.3365151197290737No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT61582.3069200107891032No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC60032.248853657805616No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA57912.1694338717894923No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT56002.097881140048551No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC55072.0630413282584588No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC54162.02895075973267No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA53912.019585218928882No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC53522.004974975274972No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA46601.7457368058261158No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC43611.633724937812809No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC43541.6311025863877484No Hit
TACCACCTAAATCTCCAACTTCTCCGAAAAAACCAACTATCCTAATAAAA40731.5258339077531693No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG40701.5247100428567146No Hit
CATAATCACCCACAACAAATTTCCCGCCGCCAAAATAAACCGCCCCCTAA40221.5067282045134416No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA39771.4898702310666228No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC38331.4359247160368027No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA37921.4205652291185902No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT37331.39846255282165No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC36721.375610633260407No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT36211.356504930020679No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA36031.3497617406419515No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC33021.237000629364342No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA32601.221266520813978No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA32211.2066562771600684No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT31441.1778104114844008No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA29841.1178709503401563No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC29251.0957682740432164No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT28811.0792849222285492No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT28541.069170138160458No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA28311.060553840620973No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA27691.0373272994275782No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA26801.0039859741660921No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC26190.9811340546048491No Hit
ACTCCCACACACACTAACACAATAAAAAAACGCTACGAACATCAATACAC24910.9331824856894537No Hit
AAACTACAAAATCAAAACCAACCCGAAACCACACCAAACCCCCACAAAAC24400.9140767824497258No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT23210.869496808223694No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA22930.8590074025234514No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT21060.7889531573111157No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACACT20350.7623550214283573No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA20140.7544879671531753No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC19930.7466209128779933No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC19440.7282644529025684No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC19400.7267659663739623No Hit
ATACAAAACAAACCCCTATCTCCTCTGCCGCGACCTTAAAAAACAATACC15030.563056313123745No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC12940.4847603920040759No Hit
CCAACTAAAACTCAACATAATATCAAACAAACCCTTCCAAATTCTTTTAA12760.47801720262534836No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCGCTTACCGATCCACTC12550.47015014835016633No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACAAAAAAAATAATCACA11030.41320766026313427No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC10080.3776186052087392No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAGCCCCCACCACCCGCAA10050.37649474031228464No Hit
CAACCATAAATACACAAAAAACAACCTATACCACCACTAAATACACAACC9830.36825306440495104No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACGAAAAAAATAATCACA8110.30381814367488835No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAACATCCCAAAAC7670.28733479186022115No Hit
CAAAACAAACAACACTAAAACCAAAACAACACCCAACTCTACAAACCCCC7590.28433781880300896No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA6820.2554919531273414No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCGCCAAAAAAACCC6520.24425330416279556No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA6480.24275481763418946No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCGAACCCTAAATCCCACC6320.23676087151976505No Hit
CAACTACCAAACCCTAAAAAACCACAAAAACAAAACTATCTCCAAACCTA5980.2240237360266131No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCACAA5750.215407438487128No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAAATCCTATAACTCTAA5110.19143165402943027No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG5070.18993316750082417No Hit
CCTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT4300.1610873018251566No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCACATCA4140.15509335571073216No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAATACCTAAAATAAA4000.1498486528606108No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAACAAAAACACCTAACGCTAA3830.14348008511403482No Hit
CCATAAAAACAAAAACCTAAAAAAACTAAAACACCTATTAACCTATCCCG3750.1404831120568226No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA3550.13299067941379206No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)