FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888151

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888151
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences256822
Sequences flagged as poor quality0
Sequence length301
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT83573.2540047192218737No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC70172.7322425648893005No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT68492.6668276082267095No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA58812.2899128579327317No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA57842.252143507954926No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA55812.1731004353209618No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC55342.1547998224451175No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC51662.01150991737468No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA50561.968678695750364No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC48921.9048212380559297No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC45771.7821681943135714No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA44311.725319481975843No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC43321.6867713825139592No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT42381.650170156762271No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA42091.6388782892431335No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA41811.6279757964660346No Hit
AAACCTATCAAAACCTCCCCACCAAAAACCTACCGCTCTAACCACCCTCG41251.6061708109118378No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT38991.518172119210971No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA38031.4807921439752045No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC36431.4184921852489274No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT35581.3853953321755925No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC35181.3698203424940232No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT35021.3635903466213954No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA34451.3413959863251592No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC34121.3285466198378644No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT33711.3125822554142557No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCGAAACCCAAAAAA33431.3016797626371572No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA33421.301290387895118No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA33211.2931135183122942No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC32591.2689722843058617No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC31881.241326677621076No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA29461.1470979900475817No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA26531.0330111906300863No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC24870.9683749834515735No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA24670.9605874886107889No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA24070.9372250040884348No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACAAAAAAAATAATCACA24010.9348887556361993No Hit
TACCACCTAAATCTCCAACTTCTCCGAAAAAACCAACTATCCTAATAAAA22860.8901106603016875No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT22400.8721994221678828No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG20850.8118463371518017No Hit
ACTCCCACACACACTAACACAATAAAACGACGCTACGAACATCAATACAC20520.798996970664507No Hit
AAAAAAAATCCCAAAACAAACATACAAAATCAAAAAAATACCTAAAATAA18600.7242370201929741No Hit
CAACTACCAAACCCTAAAAAACCACTCTACGCTAACCAAAACCCAAAAAA17590.6849101712470116No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCACCT17350.67556517743807No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC16540.644025823332892No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC16310.6350702042659897No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT14230.5540802579218291No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC12700.49450592238982644No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCGAACCCTAAATCCCGCC11880.46257719354260923No Hit
TACCACCTAAATCTCCAACTTCTCCAAAAAAACCAACTATCCTAATAAAA11730.45673657241202076No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA11050.4302590899533529No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT10680.4158522244979012No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC10140.3948259884277826No Hit
AACCACTCACCTACTTAATCTCCCAATTCCAATATATCCAAAACTTACCC9920.3862597441029196No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA7160.27879231530009113No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACACTACCTT5960.23206734625538314No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCACAC5770.22466922615663767No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACGCCAAACCCCCACAAAAC4700.18300612875843972No Hit
AAAAAAAATCCCAAAACAAACATACAAAATCAAAAAATACCTAAAATAAA4680.18222737927436125No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCACCAAAAAAACC4520.1759973834017335No Hit
CCACACTCCTAAAAACCTAACTTCCTAAAAAATTACTAACCAATTTAAAA4270.16626301485075265No Hit
CACTCCATTATTCTTATCTTTAATCTCTTTTTATTTATAAAAATCCAATT3430.13355553651945706No Hit
CCCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA3160.12304241848439774No Hit
CTCATTCAAAAAACCCAAAAAACCCAACTTTACTACTCCCTTTTCCCTTT3110.12109554477420159No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACACTAAAACCTTCATC3100.12070617003216236No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCACCAAAAAAACCC2920.11369742467545614No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAATACTAAAA2840.11058242673914229No Hit
CCTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT2830.11019305199710305No Hit
TAAAACTAAACCCCACATACCTTCCCCTCCAAATAACCCCCATAAATAAA2800.10902492777098535No Hit
CCATAAAAACAAAAACCTAAAAAAACTAAAACACCTATTAACCTATCCCG2770.10785680354486765No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA2740.10668867931874995No Hit
CCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA2680.10435243086651454No Hit
CCTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA2670.1039630561244753No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)