FastQCFastQC Report
Sat 3 Sep 2022
EGAF00000888152

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888152
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences205537
Sequences flagged as poor quality0
Sequence length301
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAAAAGAATTTGGAAGGGTTTGTGAGATATTGGATTGCGGGGTAATAA168088.177603059303191No Hit
GAATTGAAGGAGAATTTATTTGTGGTTGTTATTGTTTTATTAGGATAGTT155127.5470596534930445No Hit
GGTGGGGAATAAGGTTTTGTATTTATTGAGTTTTTGTTATAGGTTTGGGT152097.399640940560579No Hit
GAAAGGGTTTTTTGAGAAGGTTATTTTGTTTGGAGTGGGGGCGTCGGGTA120995.886531378778517No Hit
TGGTTTTTATTTTTGTGTTTTTTGGGGATGGTTTTTAAGGATAGTATGTT93354.541761337374779No Hit
TAGGTTGTTTTGGTTTAGAGAGGGCGGGAGTTTTTGTAGGATAATATAGT82083.9934415701309254No Hit
TTAGGGTGGAAGATAGAGATTTGTGTTTATAGGGTTTAGGAAGGCGGGTA74413.620272748945445No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTGT70683.4387969076127414No Hit
AGGAAGGTAGTTAAGAGGGGTATTTTTGTTTTTCGTTTGAGATTTTTTGT61733.003352194495395No Hit
AGGAAGGTAGTTAAGAGGGGTATTTTTGTTTTTTGTTTGAGATTTTTTGT60972.966375883660849No Hit
TTTTTAGGTGAGATTAGGGATTTGGGCGTGGGGGGCGGTTAAAGGTATTT54552.6540233631900825No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTGTT53932.6238584780355847No Hit
TTTGGAGAGGAGGTTTTTAGATTTTATTTTGGTGGTATAGTGTTTTTTTT50502.456978548874412No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTAATTAATTGAGAAGTTGTT41652.026399139814243No Hit
TGGGTTGTGATTAGATGGTAGAAAGGGGTTTTAGTTAGGTTGATAATGGT38921.893576339053309No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTTAATTAATTGAGAAGTTGT33931.650797666600174No Hit
TAGTTTTGGGGTTTTTAGGATTTTTTTTTTTTTTATTTTTTTTAGGTTTT28561.3895308387297665No Hit
TAGTTTTGGGGTTTTTAGGATTTTTTTTTTTTTATTTTTTTTAGGTTTTA25881.2591406899974213No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTAATTAATTGAGAAGTTGTTT25401.2357872305229716No Hit
GTGAAGTTTTTGGTTTTTTGTTGTATATGAAGGGTTTATAGGGTATGGGA24771.205135814962756No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTTG21361.0392289466130185No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTGTTT20200.9827914195497648No Hit
TAGTTTTGGGGTTTTTAGGATTTTTTTTTTTTATTTTTTTTAGGTTTTAT15490.7536355984567256No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTTTAATTAATTGAGAAGTTG12690.6174070848557681No Hit
TAGTTTTGGGGTTTTTAGGATTTTTTTTTTTTTTTATTTTTTTTAGGTTT11780.5731328179354569No Hit
GTTGGTTTTTTTGGGGTGAAGGATGTTATTGATGATATGAGGGGTTTTTT11260.5478332368381361No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTAATTAATTGAGAAGTTGTTTT10870.5288585510151457No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTTT10150.49382836180347095No Hit
TAGTTTTGGGGTTTTTAGGATTTTTTTTTTTATTTTTTTTAGGTTTTATA6580.3201370069622501No Hit
TGGTTTTTATTTTTGTGTTTTTTGGAGGAGATAGGGGTTTGTTTTGTATA4730.23012888190447464No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTGTTTT4340.2111541960814841No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTTTTAATTAATTGAGAAGTT3900.18974685822990509No Hit
GAAAGGGTTTTTTGAGAAGGTTATTTTGCTTGGAGTGGGGGCGTCGGGTA3870.18828726701275197No Hit
TAGTTTTGGGGTTTTTAGGATTTTTTTTTTTTTTTTATTTTTTTTAGGTT3590.17466441565265622No Hit
TAGTTTTGGGGTTTTTAGGATTTTTTTTTTTTTATTTTTTTTAGGTTTTT3370.1639607467268667No Hit
GATAGGTTTGGAGATAGTTTTGTTTACACTGACGACGTGGTTTTTTAGGG3050.14839177374390014No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTAATTAATTGAGAAGTTGTTTTT3000.14595912171531158No Hit
TAGTTTTGGGGTTTTTAGGATTTTTTTTTTTTTTTTTTTTTTTAGGTTTT3000.14595912171531158No Hit
TTTAGGTTGTAAGATAGAGTGGGGTTTGGAGTGTTTGGAGTGTTTGGAGT2970.14449953049815847No Hit
TAGTTTTGGGGTTTTTAGGATTTTTTTTTTTTTTTTTTTTTTTTAGGTTT2730.13282280076093356No Hit
AGGTATTAGGGAGTTGTATGGGGTTGGTTTTGATTTTGTAGTTTAGACCA2480.12065954061799092No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2290.11141546290935453No Hit
AGGTATTAGGGAGTTGTATGGGGTACACTGACGACATGGTTCTACAAGGT2280.1109289325036368No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)