FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117617
Sequences flagged as poor quality0
Sequence length151
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT1414012.022071639303842No Hit
CACACACACAAAACTATAATAAATACCCTAAAAAACACTCCGTAAAATTC1269410.79265752399738No Hit
AAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC86857.384136646913286No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA72686.179378831291395No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAATATCTAAAA67435.733014785277638No Hit
ACCAAACAACCAAACTAAACAACACTAAAAACGAAAAACCCAAATATCCT52644.475543501364598No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT51254.357363306324766No Hit
ACTCAAACTAATACACCACTACATACCGCCCCCTAAAACCCCCTCCTTCC34812.9596061793788313No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA31952.716444051455147No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA30972.6331227628659124No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT30002.5506516915071797No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACAAAAAACCCAAATATCCT22971.9529489784639975No Hit
AAATTACAAAAATAAACTAAAATCCCAACACATCAAAAACACAATAATAC21691.8441211729596914No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAATA19341.644320123791629No Hit
CTATTAACCCCCTCCCTAAACAATCACCATTTATATTATTTATTTCCTAA16541.4062592992509586No Hit
AAATTACAAAAATAAACTAAAATCCCAACGCATCAAAAATACAATAATAC13421.140991523334212No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAATA11931.0143091559893553No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAA9430.8017548483637569No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAATAA7430.6317114022632783No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAA5730.48717447307787143No Hit
ACCCCACTCCAACTATCCAAAAAAAAACTCTAAAAATAAAAAAAAAATAA5120.4353112220172254No Hit
ACTCAAACTAATACACCACTACATACCACCCCCTAAAACCCCCTCCTTCC3740.31798124420789514No Hit
CCCAAACAACCAAACTAAACAACACTAAAAACGAAAAACCCAAATATCCT3430.29162451006232093No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT3370.28652320667930653No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAT2960.25166430022870845No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAAAT2940.24996386576770363No Hit
AAAAACTAACACACACTTATACCCCCAAAACAACCATAAATACACAAAAA2780.23636039007966536No Hit
CCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAATATCTAAAA2730.23210930392715337No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAAACTCTAAAAATAAAAAAAAA2320.19725039747655526No Hit
CAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC2150.18279670455801458No Hit
ACCCCACTCCAACTATCCAAAAAAAAACTCTAAAAATAAAAAAAAATAAA2150.18279670455801458No Hit
CCTCAAACTAATACACCACTACATACCGCCCCCTAAAACCCCCTCCTTCC2050.17429453225299063No Hit
CCAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTTA2030.17259409779198587No Hit
CCCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT1770.15048844979892362No Hit
CCAAAAAAACCAACAAAAAACTAAATAAAACCGAAAACTTCTCCATCTTA1730.14708758087691406No Hit
CACACACACAAAACTATAATAAATACCCTAAAAACACTCCGTAAAATTCA1630.1385854085718901No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACAAAAAACCCAAATATCCT1520.1292330190363638No Hit
CCCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAATA1390.11818019503983268No Hit
CCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA1390.11818019503983268No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)