FastQCFastQC Report
Thu 1 Sep 2022
EGAF00000888161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43392
Sequences flagged as poor quality0
Sequence length151
%GC29

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA1052724.260232300884958No Hit
AAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC1027423.67717551622419No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA25475.8697455752212395No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT22565.199115044247788No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAATA11992.7631821533923304No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAATA8131.873617256637168No Hit
AAATTACAAAAATAAACTAAAATCCCAACGCATCAAAAATACAATAATAC6771.5601954277286136No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAA5771.3297382005899705No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAATAA4921.133849557522124No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAA4551.0485803834808258No Hit
CAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC4090.9425700589970502No Hit
CACACACACAAAACTATAATAAATACCCTAAAAAACACTCCGTAAAATTC2930.6752396755162242No Hit
ACCCCACTCCAACTATCCAAAAAAAAACTCTAAAAATAAAAAAAAAATAA2530.5830567846607669No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAAAT2150.49548303834808255No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAACAAAAAAAAAAT2020.46552359882005895No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAT2000.4609144542772861No Hit
CCCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT1920.4424778761061947No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA1590.36642699115044247No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAACAAAAAAAAATA1170.26963495575221236No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAAACTCTAAAAATAAAAAAAAA1160.26733038348082594No Hit
CAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA1080.2488938053097345No Hit
ACCCCACTCCAACTATCCAAAAAAAAACTCTAAAAATAAAAAAAAATAAA1050.2419800884955752No Hit
CCCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAATA990.22815265486725664No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAACAAAAAAAAAATA920.21202064896755163No Hit
AAATTACAAAAATAAACTAAAATCCCAACACACCAAAAATACAATAATAC850.1958886430678466No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAG740.17053834808259588No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAGCTCTAAAAATAAAAAAAAAAT710.1636246312684366No Hit
GAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC700.16132005899705015No Hit
AAATTACAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATACA690.15901548672566373No Hit
CCCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAATA680.1567109144542773No Hit
ACTCAAACTAATACACCACTACATACCACCCCCTAAAACCCCCTCCTTCC670.15440634218289087No Hit
CTATTAACCCCCTCCCTAAACAATTACCGTTTATATTATTTATTTCCTAA630.14518805309734514No Hit
CCCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAA630.14518805309734514No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAATAA560.1290560471976401No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAACAAAAAAAAAA560.1290560471976401No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAAAA550.1267514749262537No Hit
ACCCCACTCCAACTATCCAAAAAAAAACTCTAAAAATAAAAAAAAAAATA550.1267514749262537No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAACAAAAAAAAATAA540.12444690265486726No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT530.12214233038348084No Hit
CTATTAACCCCCTCCTAAACAATCACCGTTTATATTATTTATTTCCTAAT520.11983775811209439No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAGCTCTAAAAATAAAAAAAAAAA520.11983775811209439No Hit
AAATTACAAAAATAAACTAAAATCCCAATACATCAAAAATACAATAATAC490.11292404129793511No Hit
AAATTACAAAAATAAACTAAAATCCCAACACATCAAAATACAATAATACA490.11292404129793511No Hit
AAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAGTAATAC470.10831489675516225No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAACAAAAAAAAAAA450.1037057522123894No Hit
CTATTAACCCCCTCCCTAACAATCACCGTTTATATTATTTATTTCCTAAT440.10140117994100295No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)