FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888171

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888171
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107302
Sequences flagged as poor quality0
Sequence length151
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACAAAACTATAATAAATACCCTAAAAAACACTCCGTAAAATTC1289012.012823619317441No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT105439.825539132541799No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT103489.643809062272837No Hit
AAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC97229.060408939255558No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA85087.929022758196493No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA58655.465881344243351No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA36193.3727237143762467No Hit
ACTCAAACTAATACACCACTACATACCACCCCCTAAAACCCCCTCCTTCC27182.5330375948258186No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA26982.5143986132597713No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT20681.9272706939292839No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAATA16301.5190769976328493No Hit
ACTCAAACTAATACACCACTACATACCGCCCCCTAAAACCCCCTCCTTCC15601.4538405621516841No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT7950.7408995172503774No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAATA7810.7278522301541444No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAA7660.713872993979609No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAA7420.6915062161003522No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAATAA6820.6355892714022106No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAACACACAAAAA4940.4603828446813666No Hit
ACCCCACTCCAACTATCCAAAAAAAAACTCTAAAAATAAAAAAAAAATAA4120.38396302026057294No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAAAT3930.3662559877728281No Hit
CCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA2980.27772082533410375No Hit
CAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC2970.2767888762558014No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAGAAACCCAAATATCCT2640.24603455667182345No Hit
CACACACACAAAACTATAATAAATACCCTAAAAACACTCCGTAAAATTCA2520.23485116773219514No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATAAACAAAACGTCTAAAAA2510.23391921865389276No Hit
ACCCCACTCCAACTATCCAAAAAAAAACTCTAAAAATAAAAAAAAATAAA2170.20223294999161248No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAT2170.20223294999161248No Hit
CCTCAAACTAATACACCACTACATACCACCCCCTAAAACCCCCTCCTTCC2010.18732176473877465No Hit
CCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA1930.17986617211235578No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAAACTCTAAAAATAAAAAAAAA1860.17334252856423926No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCACCTT1780.16588693593782036No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCAAAAACTTCTCCACCTT1600.14911185252837786No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACCTCTCCATCTT1550.14445210713686604No Hit
CCAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTTA1480.13792846358874952No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAGTTAAACAAAACGTCTAAAA1410.13140482004063297No Hit
CCCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAATA1350.1258131255708188No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACTTCTAAAA1350.1258131255708188No Hit
CCCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT1280.11928948202270227No Hit
CCAAAAAAACCAACAAAAAACTAAATAAAACCGAAAACTTCTCCATCTTA1260.11742558386609757No Hit
CACACACACAAAACTATAATAAATACCTTAAAAAACACTCCGTAAAATTC1160.10810609308307394No Hit
CTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAAA1120.1043782967698645No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATCTATTTCCTAA1110.10344634769156213No Hit
CCTCAAACTAATACACCACTACATACCGCCCCCTAAAACCCCCTCCTTCC1090.1015824495349574No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)