FastQCFastQC Report
Thu 1 Sep 2022
EGAF00000888173

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888173
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84911
Sequences flagged as poor quality0
Sequence length151
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACAAAACTATAATAAATACCCTAAAAAACACTCCGTAAAATTC1793221.118582986892157No Hit
AAATTACAAAAATAAACTAAAATCCCAACGCATCGAAAATACAATAATAC1467617.283979696387984No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA1418016.69983865459128No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT40704.793254113130219No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAATA23982.8241335044929397No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAATA18832.2176160921435386No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAA12781.5051053455971548No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAATAA10961.2907632697765896No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAA10831.2754531215036922No Hit
CCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA8501.0010481563048368No Hit
ACCCCACTCCAACTATCCAAAAAAAAACTCTAAAAATAAAAAAAAAATAA7190.846768969862562No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAAAT4810.5664754860972077No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAT4670.5499876341110104No Hit
CAATTACAAAAATAAACTAAAATCCCAACGCATCGAAAATACAATAATAC4530.5334997821248131No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAATTAAACAAAACGTCTAAAAA3760.44281659620072783No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACTTCTAAAA3680.43339496649432935No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAAACTCTAAAAATAAAAAAAAA2970.34977800285004296No Hit
ACCCCACTCCAACTATCCAAAAAAAAACTCTAAAAATAAAAAAAAATAAA2920.34388948428354393No Hit
CCCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT2700.31798000259094816No Hit
CTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAAA2650.31209148402444914No Hit
ACCCCACTCCAACTATCCAAAAAAAGAAACTCTAAAAATAAAAAAAAAAT2440.28735970604515315No Hit
CACACACACAAAACTATAATAAATACCCTAAAAACACTCCGTAAAATTCA2010.2367184463732614No Hit
CCCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAATA2000.2355407426599616No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAAATTAAACAAAACGTCTAAA1960.23082992780676237No Hit
AAATTACAAAAATAAACTAAAATCCCAACTCATCGAAAATACAATAATAC1820.21434207582056505No Hit
TCTCTTTACATTTAATCAAAACCCAAAAAATTAAACAAAACGTCTAAAAA1570.18489948298806985No Hit
ACCCCACTCCAACTATCCAAAAAAAAACTCTAAAAATAAAAAAAAAAATA1540.18136637184817042No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAAAA1420.16723392728857273No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAATAA1410.16605622357527294No Hit
CACACACACAAAACTATAATAAATACCTTAAAAAACACTCCGTAAAATTC1360.1601677050087739No Hit
ACCCCACTCCAACTATCCAAAAAAAGAAACTCTAAAAATAAAAAAAAATA1100.12954740846297888No Hit
CCCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAATA1090.12836970474967907No Hit
CACACACACAAAACTATAATAATACCCTAAAAAACACTCCGTAAAATTCA1040.12248118618318003No Hit
TCTCTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAAA1020.12012577875658043No Hit
CCCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAA1000.1177703713299808No Hit
AAATTACAAAAATAAACTAAAATCCCAATGCATCGAAAATACAATAATAC990.116592667616681No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAGCAAAACGTCTAAAA990.116592667616681No Hit
TCTCTTTACATTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAAA970.11423726019008137No Hit
CACACACAAAACTATAATAAATACCCTAAAAAACACTCCGTAAAATTCAA880.10363792677038311No Hit
GAATTACAAAAATAAACTAAAATCCCAACGCATCGAAAATACAATAATAC850.10010481563048368No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)