FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888175

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888175
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158690
Sequences flagged as poor quality0
Sequence length151
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT1855611.693238389312496No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT1671810.535005356355159No Hit
AAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC1621810.21992564118722No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA134038.446026844791731No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA118527.4686495683407905No Hit
CACACACACAAAACTATAATAAATACCCTAAAAAACACTCCGTAAAATTC87435.509483899426555No Hit
CACACACACAAAACTATAATAAATACCCTAAAAACACTCCGTAAAATTCA67714.266809502804209No Hit
ACTCAAACTAATACACCACTACATACCACCCCCTAAAACCCCCTCCTTCC55233.4803705337450377No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA44002.7727014934778498No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT39912.514966286470477No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAATA25861.6295922868485726No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCAAAAACTTCTCCATCTT14590.9194026088600417No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAATA13890.8752914487365303No Hit
CTATTAACCCCCTCCCTAAACAATCACCATTTATATTATTTATTTCCTAA13170.8299199697523473No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAA13130.8273993320310039No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT10090.6358308652088979No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAATAA9700.6112546474257987No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAA9430.5942403428067301No Hit
ACTCAAACTAATACACCACTACATACCGCCCCCTAAAACCCCCTCCTTCC7600.478921167055265No Hit
ACCCCACTCCAACTATCCAAAAAAAAACTCTAAAAATAAAAAAAAAATAA7010.44174176066544835No Hit
CCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA6320.398260759972273No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAAAT4540.2860923813724872No Hit
CAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC4210.26529712017140333No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAT3950.2489129749826706No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACTTCTAAAA3800.2394605835276325No Hit
CCTCAAACTAATACACCACTACATACCACCCCCTAAAACCCCCTCCTTCC3220.20291133656815175No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAAACTCTAAAAATAAAAAAAAA3160.1991303799861365No Hit
CCCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT2960.186527191379419No Hit
ACCCCACTCCAACTATCCAAAAAAAAACTCTAAAAATAAAAAAAAATAAA2700.17014304619068624No Hit
CCAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTTA2620.16510177074799925No Hit
CCAAAAAAACCAACAAAAAACTAAATAAAACCGAAAACTTCTCCATCTTA2220.13989539353456423No Hit
CTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAAA2200.1386350746738925No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)