FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences140195
Sequences flagged as poor quality0
Sequence length151
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT1853313.219444345376083No Hit
CACACACACAAAACTATAATAAATACCCTAAAAAACACTCCGTAAAATTC1665811.882021470095225No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT126889.050251435500552No Hit
AAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC124028.846249866257711No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA99917.126502371696565No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA82585.890366988836977No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA44643.1841363814686683No Hit
ACTCAAACTAATACACCACTACATACCACCCCCTAAAACCCCCTCCTTCC38012.711223652769357No Hit
CTATTAACCCCCTCCCTAAACAATCACCATTTATATTATTTATTTCCTAA31092.217625450265701No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT29082.0742537180355933No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA28072.0022112058204646No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAACATCCT25071.7882235457755271No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAATA15951.1377010592389172No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAATA10120.7218517065515888No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAA9070.6469560255358607No Hit
ACTCAAACTAATACACCACTACATACCGCCCCCTAAAACCCCCTCCTTCC8740.6234173829309176No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT8670.6184243375298691No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAA6010.4286886122900246No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAATAA5720.40800313848568065No Hit
CCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA4860.3466600092727986No Hit
ACCCCACTCCAACTATCCAAAAAAAAACTCTAAAAATAAAAAAAAAATAA3670.2617782374549735No Hit
CAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC3580.2553586076536253No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACTTCTAAAA2830.20186169264239093No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAC2740.19544206284104285No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCACCTT2680.19116230964014408No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAAAT2650.1890224330396947No Hit
CCAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTTA2440.1740432968365491No Hit
CCAAAAAAACCAACAAAAAACTAAATAAAACCGAAAACTTCTCCATCTTA2250.16049074503370306No Hit
CCTCAAACTAATACACCACTACATACCACCCCCTAAAACCCCCTCCTTCC2180.15549769963265453No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAT2170.1547844074325047No Hit
CCCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT2160.15407111523235492No Hit
CACACACACAAAACTATAATAAATACCCTAAAAACACTCCGTAAAATTCA1790.12767930382681264No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAAACTCTAAAAATAAAAAAAAA1780.12696601162666285No Hit
CTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAAA1780.12696601162666285No Hit
ACCCCACTCCAACTATCCAAAAAAAAACTCTAAAAATAAAAAAAAATAAA1610.1148400442241164No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAACATCCT1580.11270016762366702No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACAAAAAACCCAAATATCCT1500.1069938300224687No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAACA1450.10342736902171974No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)