FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888183

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888183
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109833
Sequences flagged as poor quality0
Sequence length151
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACAAAACTATAATAAATACCCTAAAAAACACTCCGTAAAATTC1221211.11869838755201No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT109559.974233609206705No Hit
AAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC101259.218540875693098No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT97218.850709713838281No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA71856.541749747343695No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA63135.747817140567953No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA33873.0837726366392615No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA33513.050995602414575No Hit
ACTCAAACTAATACACCACTACATACCACCCCCTAAAACCCCCTCCTTCC29752.7086576894011816No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCAAAAACTTCTCCATCTT21511.9584277949250224No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT20471.8637385849425945No Hit
ACTCAAACTAATACACCACTACATACCGCCCCCTAAAACCCCCTCCTTCC15271.3902925350304554No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACAAAAAACCCAAATATCCT13251.2063769541030473No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAATA13201.2018245882385075No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAATA10710.9751167681844255No Hit
CTATTAACCCCCTCCCTAAACAATCACCATTTATATTATTTATTTCCTAA8130.7402146895741717No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT6790.618211284404505No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAATAA6560.597270401427622No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAA5910.5380896451886046No Hit
ACCCCACTCCAACTATCCAAAAAAAACAACTCTAAAAATAAAAAAAAAAT4840.44066901568745276No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAA3940.35872643012573635No Hit
ACCCCACTCCAACTATCCAAAAAAAAACTCTAAAAATAAAAAAAAAATAA3180.2895304689847314No Hit
CCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA2970.2704105323536642No Hit
CAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC2850.25948485427876866No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAT2790.2540220152413209No Hit
AAAAACTAACACACACTTATACCCCCAAAACAACCATAAATACACAAAAA2750.25038012254968905No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAAAT2150.19575173217521147No Hit
CCTCAAACTAATACACCACTACATACCACCCCCTAAAACCCCCTCCTTCC1960.17845274188996021No Hit
ACCCCACTCCAACTATCCAAAAAAAACAACTCTAAAAATAAAAAAAAATA1790.16297469795052488No Hit
ACCCCACTCCAACTATCCAAAAAAAAACTCTAAAAATAAAAAAAAATAAA1780.16206422477761692No Hit
CACACACACAAAACTATAATAAATACCCTAAAAACACTCCGTAAAATTCA1710.1556909125672612No Hit
CCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA1630.1484071271839975No Hit
CCCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT1570.14294428814654975No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAAACTCTAAAAATAAAAAAAAA1480.13475002959037813No Hit
CCAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTTA1380.12564529786129852No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAAATTAAACAAAACGTCTAAA1320.12018245882385076No Hit
CCAAAAAAACCAACAAAAAACTAAATAAAACCGAAAACTTCTCCATCTTA1300.11836151247803485No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACTTCTAAAA1220.11107772709477116No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)