FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888187

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888187
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136533
Sequences flagged as poor quality0
Sequence length151
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT1637911.996367178630807No Hit
CACACACACAAAACTATAATAAATACCCTAAAAAACACTCCGTAAAATTC1553511.378201606937518No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT128209.389671361502346No Hit
AAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC117798.627218328169747No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA100047.327166326089664No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA96397.059831689042209No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA35462.5971743095075914No Hit
ACTCAAACTAATACACCACTACATACCGCCCCCTAAAACCCCCTCCTTCC34922.5576234316978312No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT30482.232427325262025No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA18371.3454622691949931No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAATA17771.3015168494063705No Hit
ACTCAAACTAATACACCACTACATACCACCCCCTAAAACCCCCTCCTTCC14351.0510279566112222No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAATA11010.8063984531212234No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAA10560.7734393882797566No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT10450.7653827279851757No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAA6740.49365354895885977No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAATAA6470.4738781100539796No Hit
CCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA4950.3625497132561359No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGCCTAAAA4090.29956127822577694No Hit
ACCCCACTCCAACTATCCAAAAAAAAACTCTAAAAATAAAAAAAAAATAA3860.2827155339734716No Hit
CAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC3740.2739264500157471No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAAAT3110.22778375923769345No Hit
CCTCAAACTAATACACCACTACATACCGCCCCCTAAAACCCCCTCCTTCC2890.21167043864853186No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAT2890.21167043864853186No Hit
CCCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT2650.19409227073308286No Hit
CCAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTTA2220.16259805321790335No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAAACTCTAAAAATAAAAAAAAA2210.16186562955475967No Hit
CCAAAAAAACCAACAAAAAACTAAATAAAACCGAAAACTTCTCCATCTTA2060.15087927460760403No Hit
CACACACACAAAACTATAATAAATACCCTAAAAACACTCCGTAAAATTCA1590.11645536243984972No Hit
ACCCCACTCCAACTATCCAAAAAAAAACTCTAAAAATAAAAAAAAATAAA1560.11425809145041857No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAGCATCTAAAA1470.10766627848212519No Hit
CCTCAAACTAATACACCACTACATACCACCCCCTAAAACCCCCTCCTTCC1410.10327173650326293No Hit
CTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAAA1370.10034204185068811No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)