FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888191

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888191
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137121
Sequences flagged as poor quality0
Sequence length151
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACAAAACTATAATAAATACCCTAAAAAACACTCCGTAAAATTC1676912.229344885174408No Hit
AAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC135229.861363321446023No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT128779.39097585344331No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT117248.550112674207453No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCAAAAACTTCTCCATCTT74625.441908970908906No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA68224.975167917386834No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA54363.964381823353097No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA48643.5472320067677456No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA44913.2752094865119132No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT26651.943538918181752No Hit
ACTCAAACTAATACACCACTACATACCGCCCCCTAAAACCCCCTCCTTCC25301.8450857272044399No Hit
CTATTAACCCCCTCCCTAAACAATCACCATTTATATTATTTATTTCCTAA20721.5110741607777072No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAATA19361.411891686904267No Hit
ACTCAAACTAATACACCACTACATACCGCCCCCTGGAACCCCCTCCTTCC18011.313438495926955No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAATA11360.8284653700016773No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT9120.6651060012689523No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAATAA8970.6541667578270287No Hit
ACCAAACAACCAAACTAAACAACGCTAAAATCGAAAAACCCAAATATCCT8760.6388518170083357No Hit
ACTCAAACTAATACACCACTACATACCGCCCCCTGAAACCCCCTCCTTCC8600.6271832906702839No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAA7200.5250836852123307No Hit
ACTCAAACTAATACACCACTACATACCACCCCCTAAAACCCCCTCCTTCC6520.47549244827561055No Hit
ACCCCACTCCAACTATCCAAAAAAAAACTCTAAAAATAAAAAAAAAATAA5360.39089563232473507No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAA4920.35880718489509267No Hit
CCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA4200.3062988163738596No Hit
CAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC3780.27566893473647364No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAAAT3230.2355583754494206No Hit
CCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA2810.20492849381203462No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAT2770.20201136222752167No Hit
ACCCCACTCCAACTATCCAAAAAAAAACTCTAAAAATAAAAAAAAATAAA2610.19034283588946987No Hit
CCCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT2320.16919363190175102No Hit
CACACACACAAAACTATAATAAATACCCTAAAAACACTCCGTAAAATTCA2120.15460797397918627No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAATTAAACAAAACATCTAAAAA2050.1495029937062886No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAAACTCTAAAAATAAAAAAAAA1960.14293944764113448No Hit
CCAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTTA1630.11887311206890264No Hit
CCCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAATA1540.11230956600374851No Hit
CCTCAAACTAATACACCACTACATACCGCCCCCTAAAACCCCCTCCTTCC1510.1101217173153638No Hit
CAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA1490.10866315152310733No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACTTCTAAAA1380.10064103966569672No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)