FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888195

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888195
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108056
Sequences flagged as poor quality0
Sequence length151
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACAAAACTATAATAAATACCCTAAAAAACACTCCGTAAAATTC1272411.775375731102391No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT1138710.538054342192938No Hit
AAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC108049.998519286295995No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT104549.674613163544828No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA77047.129636484785666No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA57785.3472273635892495No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA38183.53335307618272No Hit
CTATTAACCCCCTCCCTAAACAATCACCATTTATATTATTTATTTCCTAA36973.421374102317317No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT25652.3737691567335455No Hit
ACTCAAACTAATACACCACTACATACCGCCCCCTAAAACCCCCTCCTTCC24652.2812245502332127No Hit
ACTCAAACTAATACACCACTACATACCACCCCCTAAAACCCCCTCCTTCC20641.9101206781668763No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAATA13541.253053972014511No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAATA10550.9763455985785149No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAA8120.7514622047827052No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT7100.6570667061523655No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAATAA5940.5497149626119789No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAA4920.4553194639816392No Hit
CCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA4120.3812837787813726No Hit
CACACACACAAAACTATAATAAATACCCTGAAAAACACTCCGTAAAATTC4080.37758199452135927No Hit
ACCCCACTCCAACTATCCAAAAAAAAACTCTAAAAATAAAAAAAAAATAA2970.27485748130598947No Hit
CAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC2960.2739320352409862No Hit
CCAAAAAAACCAACAAAAAAACTAACAAAAAAACCACTCACCTACTTAAT2840.26282668246094615No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAT2620.24246686903087286No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACTTCTAAAA2360.21840527134078624No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAAACTCTAAAAATAAAAAAAAA2260.20915081069075295No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAAAT2080.192492781520693No Hit
CCCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT2040.18879099726067963No Hit
ACCCCACTCCAACTATCCAAAAAGAAAAACTCTAAAAATAAAAAAAAAAT1750.16195306137558305No Hit
ACCCCACTCCAACTATCCAAAAAAAAACTCTAAAAATAAAAAAAAATAAA1650.15269860072554972No Hit
AAATTACAAAAATAAACTAAAATCCCAACGCATCGAGAATACAATAATAC1590.14714592433552973No Hit
CCTCAAACTAATACACCACTACATACCGCCCCCTAAAACCCCCTCCTTCC1580.1462204782705264No Hit
CCAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTTA1530.14159324794550973No Hit
CACACACACAAAACTATAATAAATACCCTAAAAACACTCCGTAAAATTCA1520.1406678018805064No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAGA1510.13974235581550307No Hit
CCAAAAAAACCAACAAAAAACTAAATAAAACCGAAAACTTCTCCATCTTA1460.13511512549048643No Hit
CAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA1330.12308432664544311No Hit
AAATTACAAAAATAAACTAAAATCCCAACGCATCAAAAATACAATAATAC1310.12123343451543643No Hit
CTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAAA1280.11845709632042645No Hit
CCTCAAACTAATACACCACTACATACCACCCCCTAAAACCCCCTCCTTCC1250.11568075812541645No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)