FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888197

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888197
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences150819
Sequences flagged as poor quality0
Sequence length151
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACAAAACTATAATAAATACCCTAAAAAACACTCCGTAAAATTC1884312.493783939689296No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCAAAAACTTCTCCATCTT1778711.793606906291648No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA139289.234910720797778No Hit
AAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC138729.197780120541841No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT119527.924730968909753No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA106267.045531398563841No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA51073.386178134054728No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT31182.067378778535861No Hit
ACTCAAACTAATACACCACTACATACCGCCCCCTAAAACCCCCTCCTTCC28061.8605082913956466No Hit
ACTCAAACTAATACACCACTACATACCACCCCCTAAAACCCCCTCCTTCC25491.6901053580782261No Hit
ACCAAACAACCAAACTAAACAACACTAAAAACGAAAAACCCAAATATCCT20671.3705169773039205No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAATA17501.1603312579979976No Hit
CTATTAACCCCCTCCCTAAACAATCACCATTTATATTATTTATTTCCTAA15611.035015482134214No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACAAAAAACCCAAATATCCT12770.8467102951219673No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAA11860.7863730697060716No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAATA11770.7804056518077961No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT8150.5403828430104961No Hit
CCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA7750.5138609856848275No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAATAA6840.4535237602689316No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAA6710.44490415663808935No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT4480.2970448020474874No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAAACTCTAAAAATAAAAAAAAA4360.2890882448497868No Hit
CAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC4080.27052294472181887No Hit
ACCCCACTCCAACTATCCAAAAAAAAACTCTAAAAATAAAAAAAAAATAA3980.26389248039040175No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAT3500.23206625159959954No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACTTCTAAAA3430.22742492656760752No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAAAT2930.1942726049105219No Hit
CTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAAA2580.17106597975056193No Hit
CCCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT2520.16708770115171165No Hit
ACTCAAACTAATACACCACTACATACCACCCCCTAAAGCCCCCTCCTTCC2350.15581591178830254No Hit
CCAAAAAACCAACAAAAAAACTAAATAAAACCAAAAACTTCTCCATCTTA2270.15051154032316882No Hit
CCAAAAAAACCAACAAAAAACTAAATAAAACCAAAAACTTCTCCATCTTA2130.14122889025918486No Hit
CCTCAAACTAATACACCACTACATACCGCCCCCTAAAACCCCCTCCTTCC2010.13327233306148428No Hit
CACACACACAAAACTATAATAAATACCCTAAAAACACTCCGTAAAATTCA2000.1326092866283426No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAAATTAAACAAAACGTCTAAA1810.12001140439865003No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCACAAATACACAAAAA1760.11669617223294149No Hit
CAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA1630.1080765686020992No Hit
CCTCAAACTAATACACCACTACATACCACCCCCTAAAACCCCCTCCTTCC1590.10542438286953236No Hit
CACACACACAAAACTATAATAAATACCTTAAAAAACACTCCGTAAAATTC1570.10409829000324893No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)