FastQCFastQC Report
Thu 1 Sep 2022
EGAF00000888201

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888201
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30943
Sequences flagged as poor quality0
Sequence length151
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACAAAACTATAATAAATACCCTAAAAAACACTCCGTAAAATTC26818.664318262611898No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA22797.365155285524998No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT22187.1680186148725085No Hit
AAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC19846.411789419254759No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA19246.21788449730149No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT17845.765439679410529No Hit
CACACACACAAAACTATAATAAATACCCTAAAAAACACTCCATAAAATTC11803.813463465080955No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA8802.8439388553146108No Hit
ACCAAACAACCAAACTAAACAACACTAAAAACGAAAAACCCAAATATCCT8612.7825356300294093No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA8562.7663768865333034No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT7092.2913098277477943No Hit
AAATTACAAAAATAAACTAAAATCCCAACACATCGAAAATACAATAATAC6402.068319167501535No Hit
CTATTAACCCCCTCCCTAAACAATCACCATTTATATTATTTATTTCCTAA6262.023074685712439No Hit
ACTCAAACTAATACACCACTACATACCGCCCCCTAAAACCCCCTCCTTCC6161.9907571987202273No Hit
ACTCAAACTAATACACCACTACATACCACCCCCTAAAACCCCCTCCTTCC3921.2668454900946904No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAATA3821.2345280031024788No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAATA2760.8919626409850371No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAA2280.7368387034224219No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT2180.7045212164302104No Hit
CCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA1770.5720195197621433No Hit
AAAAACTAACACACACTTATACCCCCAAAACAACCATAAATACACAAAAA1620.5235432892738261No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAATAA1580.5106162944769415No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAA1520.49122580228161455No Hit
CAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC990.3199431212228937No Hit
ACCCCACTCCAACTATCCAAAAAAAAACTCTAAAAATAAAAAAAAAATAA940.30378437772678796No Hit
CCTCAAACTAATACACCACTACATACCGCCCCCTAAAACCCCCTCCTTCC790.25530814723847073No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAT780.2520763985392496No Hit
CCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA720.23268590634392272No Hit
CCCAAACAACCAAACTAAACAACACTAAAAACGAAAAACCCAAATATCCT670.21652716284781695No Hit
CCCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT660.21329541414859582No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAAAT600.19390492195326892No Hit
CCTCAAACTAATACACCACTACATACCACCCCCTAAAACCCCCTCCTTCC520.16805093235949972No Hit
CTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAAA510.1648191836602786No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAAACTCTAAAAATAAAAAAAAA440.14219694276573053No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAGAACTTCTCCATCTT430.1389651940665094No Hit
CAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA430.1389651940665094No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACTTCTAAAA390.1260381992696248No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCGAATATCCT380.12280645057040364No Hit
CCCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAATA350.1131112044727402No Hit
CCCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAATA320.10341595837507675No Hit
ACCCCACTCCAACTATCCAAAAAAAAACTCTAAAAATAAAAAAAAATAAA320.10341595837507675No Hit
CCAAAAAAACCAACAAAAAAACTAACAAAAAAACCAACAAAAAAACCAAC310.1001842096758556No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)