FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888207

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888207
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137598
Sequences flagged as poor quality0
Sequence length151
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACAAAACTATAATAAATACCCTAAAAAACACTCCGTAAAATTC1503210.924577392113259No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT1476910.733440893036237No Hit
AAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC1402310.19128185002689No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT118348.600415703716624No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA105857.6926990217881075No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA72825.292228084710533No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA49393.5894417070015554No Hit
ACTCAAACTAATACACCACTACATACCGCCCCCTAAAACCCCCTCCTTCC46393.371415282198869No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT34342.4956758092414133No Hit
CACACACACAAAACTATAATAAATACCCTAAAAAACACTCCATAAAATTC28672.0836058663643366No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAATA20831.5138301428799839No Hit
ACTCAAACTAATACACCACTACATACCACCCCCTAAAACCCCCTCCTTCC16061.167168127443713No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAATA12240.8895478131949592No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAA11400.8285004142502072No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT10860.7892556577857236No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAATAA7550.5486998357534267No Hit
ACCCCACTCCAACTATCCAAAAAAAAACTCTAAAAATAAAAAAAAAATAA7500.5450660620067153No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAA7440.5407055335106615No Hit
CCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA6770.49201296530472827No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAGCCGAAAACTTCTCCATCTT6150.4469541708455065No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTACTCCATCTT5730.4164304713731304No Hit
CAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC4750.3452085059375863No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA4680.3401212226921903No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAGAAACCCAAATATCCT4390.31904533496126397No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAAAT3420.24855012427506215No Hit
CCTCAAACTAATACACCACTACATACCGCCCCCTAAAACCCCCTCCTTCC3140.2282009912934781No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAT3040.22093344380005522No Hit
CCCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT2740.1991308013197866No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACTTCTAAAA2640.19186325382636377No Hit
ACCCCACTCCAACTATCCAAAAAAAAACTCTAAAAATAAAAAAAAATAAA2620.19040974432767918No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAAACTCTAAAAATAAAAAAAAA2220.1613395543539877No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAATTAAACAAAACGTCTAAAAA2060.14971147836451112No Hit
CTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAAA1980.1438974403697728No Hit
CACACACACAAAACTATAATAAATACCCTAAAAACACTCCGTAAAATTCA1910.1388101571243768No Hit
CAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA1650.11991453364147735No Hit
AAATTACAAAAATAAACTAAAATCCCAACACATCAAAGATACAATAATAC1570.11410049564673905No Hit
GCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT1570.11410049564673905No Hit
CCAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTTA1550.11264698614805449No Hit
CCCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAATA1450.1053794386546316No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)