FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888209

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888209
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165664
Sequences flagged as poor quality0
Sequence length151
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT2411614.557175970639365No Hit
CACACACACAAAACTATAATAAATACCCTAAAAAACACTCCGTAAAATTC2094812.644871547228123No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT149929.049642650183504No Hit
AAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC136538.241380142939926No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAATATCTAAAA105746.382798918292447No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA53683.240293606335716No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA38792.3414863820745606No Hit
CTATTAACCCCCTCCCTAAACAATCACCATTTATATTATTTATTTCCTAA33041.994398300173846No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT31381.8941954800077265No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA26661.6092814371257484No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA25361.530809349043848No Hit
ACTCAAACTAATACACCACTACATACCGCCCCCTGAAACCCCCTCCTTCC25351.530205717597064No Hit
ACTCAAACTAATACACCACTACATACCGCCCCCTAAAACCCCCTCCTTCC19511.1776849526752946No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAATA18261.102231021827313No Hit
ACTCAAACTAATACACCACTACATACCACCCCCTAAAACCCCCTCCTTCC14550.8782837550705043No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAATA14420.8704365462623141No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT11110.6706345373768592No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAA10370.6259658103148541No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAATAA8420.5082576781920031No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAA7640.4611744253428626No Hit
CCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAATATCTAAAA5530.33380819007146995No Hit
ACCCCACTCCAACTATCCAAAAAAAAACTCTAAAAATAAAAAAAAAATAA4670.28189588564805873No Hit
CAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC4230.25533610198956924No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAT4180.25231794475565No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACAAAAAACCCAAATATCCT3540.2136855321614835No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAAAT3320.20040564033223873No Hit
CCAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTTA3170.191351168630481No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCCCCATCTT2760.16660227931234306No Hit
CCCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT2690.1623768591848561No Hit
CCAAAAAAACCAACAAAAAACTAAATAAAACCGAAAACTTCTCCATCTTA2620.15815143905736911No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAAACTCTAAAAATAAAAAAAAA2540.15332238748309832No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAATTAAACAAAATATCTAAAAA2330.14064612710063742No Hit
CACACACACAAAACTATAATAAATACCCTAAAAACACTCCGTAAAATTCA2320.14004249565385357No Hit
ACCCCACTCCAACTATCCAAAAAAAAACTCTAAAAATAAAAAAAAATAAA2110.12736623527139268No Hit
CTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAATATCTAAAAA2040.12314081514390574No Hit
CAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA1720.10382460884682247No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)