FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136244
Sequences flagged as poor quality0
Sequence length151
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA1365410.02172572737148No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT127919.388303338128653No Hit
AAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC117488.622765039194388No Hit
CACACACACAAAACTATAATAAATACCCTAAAAAACACTCCATAAAATTC102567.527670943307594No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT101447.445465488388479No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA76655.625935821026981No Hit
CACACACACAAAACTATAATAAATACCCTAAAAAACACTCCGTAAAATTC70725.190687296321306No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA59014.331199906050909No Hit
ACTCAAACTAATACACCACTACATACCACCCCCTAAAACCCCCTCCTTCC50673.719062857813922No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA36212.6577317166260532No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT26951.9780687589912218No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAATA17491.2837262558351195No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAATA14221.043715686562344No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAGTGCACAAAGG11700.8587534129943336No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT10230.7508587534129944No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAA8080.5930536390593347No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAATAA7980.5857138662986994No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAA5500.4036875018349432No Hit
CCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA4550.3339596606089075No Hit
ACCCCACTCCAACTATCCAAAAAAAAACTCTAAAAATAAAAAAAAAATAA4290.3148762514312557No Hit
CCTCAAACTAATACACCACTACATACCACCCCCTAAAACCCCCTCCTTCC3980.2921229558732862No Hit
ACTCAAACTAATACACCACTACATACCGCCCCCTGAAACCCCCTCCTTCC3860.28331522856052377No Hit
CAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC3830.28111329673233315No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAT3580.2627638648307448No Hit
CCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA3070.22533102375150463No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAAAT2430.17835647808343852No Hit
CCCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT2420.177622500807375No Hit
CACACACACAAAACTATAATAAATACCCTAAAAACACTCCGTAAAATTCA2360.1732186371509938No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAAATTAAACAAAACATCTAAA2340.17175068259886675No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACTTCTAAAA2300.1688147734946126No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAAACTCTAAAAATAAAAAAAAA2090.1534012506972784No Hit
ACCCCACTCCAACTATCCAAAAAAAAACTCTAAAAATAAAAAAAAATAAA1900.1394556824520713No Hit
CTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAAA1670.12257420510261002No Hit
ACTCAAACTAATACACCACTACATACCACCCCCTAAAAACCCCTCCTTCC1640.12037227327441943No Hit
CCCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAATA1610.11817034144622882No Hit
AAAAACTAACACACACTTATACCCCCAAAACAACCATAAATACACAAAAA1570.1152344323419747No Hit
CCAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTTA1400.10275681864889462No Hit
CCCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAATA1370.10055488682070403No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)