FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888213

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888213
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160304
Sequences flagged as poor quality0
Sequence length151
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT1988212.402684898692485No Hit
CACACACACAAAACTATAATAAATACCCTAAAAAACACTCCGTAAAATTC1772811.058987922946402No Hit
AAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC128678.026624413614133No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT127127.929933127058589No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA81545.08658548757361No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA72544.525152210799481No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA68564.276873939514922No Hit
CTATTAACCCCCTCCCTAAACAATCACCATTTATATTATTTATTTCCTAA52543.2775227068569714No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA41632.5969408124563333No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT34742.1671324483481387No Hit
ACTCAAACTAATACACCACTACATACCACCCCCTAAAACCCCCTCCTTCC28221.7604052300628805No Hit
ACTCAAACTAATACACCACTACATACCGCCCCCTAAAACCCCCTCCTTCC21191.3218634594270886No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAATA18971.1833765844894701No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCAAAAACTTCTCCATCTT14130.8814502445353829No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAATA13680.8533785806966763No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAA11230.700543966463719No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT10880.6787104501447251No Hit
AAAAACTAACACACACTTATACCCCCAAAACAACCATAAATACACAAAAA9950.6206956782113984No Hit
ACTCAAACTAATACACCACTACATACCGCCCCCTAGAACCCCCTCCTTCC8820.5502046112386466No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAA8590.5358568719433078No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAATAA7510.4684848787304122No Hit
ACCCCACTCCAACTATCCAAAAAAAAACTCTAAAAATAAAAAAAAAATAA4320.269487972851582No Hit
CAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC4160.2595069368200419No Hit
CCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA4020.25077353029244437No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAT3970.2476544565325881No Hit
CCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA3670.22894001397345043No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAAAT3530.22020660744585285No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAAACTCTAAAAATAAAAAAAAA2980.1858967960874339No Hit
CCAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTTA2960.18464916658349137No Hit
CCCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT2700.16842998303223874No Hit
CCAAAAAAACCAACAAAAAACTAAATAAAACCGAAAACTTCTCCATCTTA2540.15844894700069867No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAAATTAAACAAAACATCTAAA2530.15782513224872743No Hit
CCTCAAACTAATACACCACTACATACCACCCCCTAAAACCCCCTCCTTCC2390.14909172572112986No Hit
CACACACACAAAACTATAATAAATACCCTAAAAACACTCCGTAAAATTCA2120.132248727417906No Hit
ACCCCACTCCAACTATCCAAAAAAAAACTCTAAAAATAAAAAAAAATAAA1860.11602954386665336No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACTTCTAAAA1760.10979139634694081No Hit
CCTCAAACTAATACACCACTACATACCGCCCCCTAAAACCCCCTCCTTCC1740.10854376684299831No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTGAACAAAACATCTAAAA1660.10355324882722827No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCGTCTT1640.10230561932328575No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)