FastQCFastQC Report
Thu 1 Sep 2022
EGAF00000888215

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888215
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28030
Sequences flagged as poor quality0
Sequence length151
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACAAAACTATAATAAATACCCTAAAAAACACTCCGTAAAATTC324211.56617909382804No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT288510.29254370317517No Hit
AAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC23048.219764537995006No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA22688.091330717088834No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT22407.991437745272922No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA14155.0481626828398145No Hit
CTATTAACCCCCTCCCTAAACAATCACCATTTATATTATTTATTTCCTAA11474.09204423831609No Hit
ACTCAAACTAATACACCACTACATACCACCCCCTAAAACCCCCTCCTTCC11123.9671780235462006No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA9493.385658223332144No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT6612.3581876560827686No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACAAAAAACCCAAATATCCT4181.4912593649661077No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAATA3721.3271494826971102No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA3201.1416339636104174No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAATA3141.120228326792722No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAA2250.8027113806635746No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT1780.6350338922582948No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAATAA1610.5743845879414913No Hit
CCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA1590.5672493756689262No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAA1250.4459507670353193No Hit
CAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC1220.43524794862647165No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAT1020.36389582590082054No Hit
CCTCAAACTAATACACCACTACATACCACCCCCTAAAACCCCCTCCTTCC950.3389225829468427No Hit
ACCCCACTCCAACTATCCAAAAAAAAACTCTAAAAATAAAAAAAAAATAA920.328219764537995No Hit
AAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAACAATAC870.31038173385658224No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAAAT730.2604352479486265No Hit
AAATTACAAAAATAAACTAAAATCCCAACACATAAAAAATACAATAATAC640.22832679272208348No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAAACTCTAAAAATAAAAAAAAA620.22119158044951837No Hit
CCCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT600.21405636817695325No Hit
CTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAAA600.21405636817695325No Hit
AAATTACAAAAATAAACTAAAATCCCAATACATCAAAAATACAATAATAC550.1962183374955405No Hit
CCAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTTA420.14983945772386728No Hit
CACACACACAAAACTATAATAAATACCCTAAAAACACTCCGTAAAATTCA400.14270424545130217No Hit
CCAAAAAAACCAACAAAAAACTAAATAAAACCGAAAACTTCTCCATCTTA390.13913663931501963No Hit
CAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA380.13556903317873706No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACAAAAAACCCAAATATCCT370.13200142704245452No Hit
CACACACACAAAACTATAATAAATACCCTAAAAAACACTCCGTAAGATTC350.1248662147698894No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAATAA340.12129860863360686No Hit
GAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC340.12129860863360686No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)