FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888223

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888223
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113063
Sequences flagged as poor quality0
Sequence length151
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACAAAACTATAATAAATACCCTAAAAAACACTCCGTAAAATTC1856716.421817924519956No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT1297811.47855620317876No Hit
AAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC1227510.856778963940458No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA73376.4893024243121085No Hit
ACTCAAACTAATACACCACTACATACCACCCCCTAAAACCCCCTCCTTCC62495.527007066856531No Hit
CTATTAACCCCCTCCCTAAACAATCACCATTTATATTATTTATTTCCTAA55314.891962887947428No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA42123.7253566595614838No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT33802.989483739154277No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAATA19291.7061284416652662No Hit
AAATTACAAAAATAAACTAAAATCCCAACACATCGAAAATACAATAATAC17711.5663833437994747No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAATA12951.1453791249126595No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAA12161.0755065759797635No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT11741.038359144901515No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAA8460.7482553974332894No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAATAA8410.7438330842096884No Hit
CCTCAAACTAATACACCACTACATACCACCCCCTAAAACCCCCTCCTTCC4960.43869347178121926No Hit
CAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC4930.4360400838470588No Hit
ACCCCACTCCAACTATCCAAAAAAAAACTCTAAAAATAAAAAAAAAATAA4610.4077372792160123No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAAAT4040.3573229084669609No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAT3470.3069085377179095No Hit
CCCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT3180.2812591210210237No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAAACTCTAAAAATAAAAAAAAA3100.27418341986326206No Hit
ACCCCACTCCAACTATCCAAAAAAAAACTCTAAAAATAAAAAAAAATAAA2480.21934673589060963No Hit
ACTCAAACTAATACACCACTACATACCACCCCCTAAAAACCCCTCCTTCC2010.17777699158876026No Hit
CACACACACAAAACTATAATAAATACCCTAAAAACACTCCGTAAAATTCA1990.17600806629931987No Hit
CCCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAATA1770.15654988811547543No Hit
CACACACACAAAACTATAATAAATACCTTAAAAAACACTCCGTAAAATTC1700.15035864960243403No Hit
CAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA1490.13178493406330985No Hit
CACACACACAAAACTATAATAAATACCCTAAAAAAACACTCCGTAAAATT1450.12824708348442904No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAAAA1330.11763353174778664No Hit
GCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT1330.11763353174778664No Hit
CCCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAATA1300.11498014381362603No Hit
CTATTAACCCCCTCCCTAAACAATCCCTTCTCATTAACCCAATAAAACTG1210.10701998001114424No Hit
CCCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAA1140.10082874149810284No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)