FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888225

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888225
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165225
Sequences flagged as poor quality0
Sequence length151
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACAAAACTATAATAAATACCCTAAAAAACACTCCGTAAAATTC2237713.543349977303675No Hit
AAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC1726410.4487819639885No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT1666410.085640792858223No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA135438.196701467695567No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA107106.482069904675443No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA68964.1737025268573165No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA62223.765773944620971No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT45422.748978665456196No Hit
ACTCAAACTAATACACCACTACATACCGCCCCCTAAAACCCCCTCCTTCC36182.18974126191557No Hit
CTATTAACCCCCTCCCTAAACAATCACCATTTATATTATTTATTTCCTAA33462.0251172643365107No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAATA27241.6486609169314572No Hit
CTATTAACCCCCTCCCTAAACAATCACCATTTACATTATTTATTTCCTAA21801.3194129217733395No Hit
ACTCAAACTAATACACCACTACATACCGCCCCCTGAAACCCCCTCCTTCC19011.1505522771977605No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAATA16410.9931911030413073No Hit
ACTCAAACTAATACACCACTACATACCACCCCCTAAAACCCCCTCCTTCC15290.9254047510969889No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT14810.8963534574065669No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAA14380.8703283401422303No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT11980.7250718716901196No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAATAA9210.5574216976849751No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAA8950.5416855802693297No Hit
CCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA6750.40853381752156154No Hit
ACCCCACTCCAACTATCCAAAAAAAAACTCTAAAAATAAAAAAAAAATAA6250.3782720532607051No Hit
CAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC5520.33408987743985474No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTTCTAA5220.3159328188833409No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAAAT4720.2856710546224845No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAT4690.2838553487668331No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAAACTCTAAAAATAAAAAAAAA3660.2215161143894689No Hit
CCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA3650.2209108791042518No Hit
CCCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT3390.20517476168860643No Hit
CCTCAAACTAATACACCACTACATACCGCCCCCTAAAACCCCCTCCTTCC2460.14888788016341353No Hit
CACACACACAAAACTATAATAAATACCCTAAAAACACTCCGTAAAATTCA2450.1482826448781964No Hit
CTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAAA2270.1373884097442881No Hit
ACCCCACTCCAACTATCCAAAAAAAAACTCTAAAAATAAAAAAAAATAAA2180.13194129217733394No Hit
CAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA2100.12709940989559693No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACTTCTAAAA1960.11862611590255712No Hit
CCCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAATA1870.11317899833560296No Hit
CACACACACAAAACTATAATAAATACCTTAAAAAACACTCCGTAAAATTC1690.10228476320169465No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)