FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888229

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888229
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences152176
Sequences flagged as poor quality0
Sequence length151
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACACACAAAACTATAATAAATACCCTAAAAAACACTCCGTAAAATTC1664210.9360214488487No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA1527910.040348018084323No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCAAAAACTTCTCCATCTT135198.883792450846387No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT122278.03477552307854No Hit
AAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC120177.896777415624014No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA105166.910419514246661No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT64814.258884449584691No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA49443.248869729786563No Hit
CTATTAACCCCCTCCCTAAACAATCACCATTTATATTATTTATTTCCTAA39932.623935443171065No Hit
ACTCAAACTAATACACCACTACATACCACCCCCTAAAACCCCCTCCTTCC35112.3071969298706763No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT28361.863631584481127No Hit
ACTCAAACTAATACACCACTACATACCGCCCCCTAAAACCCCCTCCTTCC19371.27286825780675No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAATA17951.1795552518136894No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAATA11680.767532330985175No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAA10200.6702765219219851No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT10010.6577909788665756No Hit
CCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA9780.6426769004310798No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAATAA7350.4829933760908422No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAA5390.3541951424666176No Hit
ACTCAAACTAATACACCACTACATACCGCCCCCTGGAACCCCCTCCTTCC5210.3423667332562296No Hit
ACCCCACTCCAACTATCCAAAAAAAAACTCTAAAAATAAAAAAAAAATAA4380.28782462411944065No Hit
CAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC4360.28651035642939754No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACTTCTAAAA3810.2503679949532121No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAT3290.21619703501209128No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA3200.2102828304068973No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAAAT3120.20502575964672487No Hit
CTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAAA3050.20042582273157397No Hit
CACACACACAAAACTATAATAAATACCCTAAAGAACACTCCGTAAAATTC2990.19648301966144463No Hit
CACACACACAAAACTATAATAAATACCCTAAAAACACTCCGTAAAATTCA2860.18794027967616445No Hit
CCTCAAACTAATACACCACTACATACCACCCCCTAAAACCCCCTCCTTCC2730.17939753969088423No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAAACTCTAAAAATAAAAAAAAA2670.1754547366207549No Hit
CCCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT2500.16428346125538848No Hit
CACACACACAAAACTATAATAAATACCCTAAAAAACACTCCGTAAAGTTC2280.1498265166649143No Hit
CAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA2130.139969508989591No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAATTAAACAAAACGTCTAAAAA2060.13536957207444011No Hit
ACCCCACTCCAACTATCCAAAAAAAAACTCTAAAAATAAAAAAAAATAAA1980.1301125013142677No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAAATTAAACAAAACGTCTAAA1960.1287982336242246No Hit
CCAAAAAAACCAACAAAAAACTAAATAAAACCAAAAACTTCTCCATCTTA1840.12091262748396593No Hit
CCAAAAAACCAACAAAAAAACTAAATAAAACCAAAAACTTCTCCATCTTA1780.1169698244138366No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCAAAAACTTCTCCATCCT1700.11171275365366418No Hit
CCTCAAACTAATACACCACTACATACCGCCCCCTAAAACCCCCTCCTTCC1660.10908421827357798No Hit
CCCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAATA1620.10645568289349173No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)