FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177596
Sequences flagged as poor quality0
Sequence length151
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT2422213.638820694159778No Hit
CACACACACAAAACTATAATAAATACCCTAAAAAACACTCCGTAAAATTC1991411.21309038491858No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA169059.518795468366404No Hit
AAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC151528.53172368747044No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA149738.43093312912453No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT70633.977003986576274No Hit
ACTCAAACTAATACACCACTACATACCGCCCCCTAAAACCCCCTCCTTCC59873.3711344850109235No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA54253.054685916349467No Hit
ACCAAACAACCAAACTAAACAACACTAAAAACGAAAAACCCAAATATCCT52832.9747291605666795No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATCTATTTCCTAA28731.6177166152390818No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT22941.291695758913489No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAATA21251.1965359580170725No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA11590.652604788396135No Hit
CCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA11110.6255771526385729No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAATA9920.5585711389896169No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAATAA7820.44032523255028266No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAA7120.4009099304038379No Hit
ACCCCACTCCAACTATCCAAAAAAAAACTCTAAAAATAAAAAAAAAATAA5850.32939931079528817No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAA5770.3248947048356945No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT5720.3220793261109484No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAACAAAAAAAAAAT5060.2849163269443005No Hit
CAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC4810.2708394333205703No Hit
CCTCAAACTAATACACCACTACATACCGCCCCCTAAAACCCCCTCCTTCC4480.2522579337372463No Hit
CCCAAACAACCAAACTAAACAACACTAAAAACGAAAAACCCAAATATCCT4160.23423950989887157No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACTTCTAAAA4090.2302979796842271No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAAAT3810.21453185882564924No Hit
CTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAAA3510.19763958647717292No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAACAAAAAAAAAATA3360.18919345030293475No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAAATTAAACAAAACGTCTAAA3310.1863780715781887No Hit
CCAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTTA3270.18412576859839186No Hit
CCAAAAAAACCAACAAAAAACTAAATAAAACCGAAAACTTCTCCATCTTA3090.17399040518930606No Hit
CACACACACAAAACTATAATAAATACCCTAAAAACACTCCGTAAAATTCA2820.15878736007567737No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAT2600.14639969368679476No Hit
ACCCCACTCCAACTATCCAAAAAAAAACTCTAAAAATAAAAAAAAATAAA2460.1385166332575058No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAACAAAAAAAAATA2090.11768283069438501No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAATTAAACAAAACGTCTAAAAA2090.11768283069438501No Hit
CAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA1960.11036284601004527No Hit
CCCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT1900.10698439154035No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAACAAAAAAAAATAA1780.10022748260095947No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)