FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888232

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888232
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179143
Sequences flagged as poor quality0
Sequence length151
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAAGGTTTGGGGTTATTTTAGTGGAGTCGGAGGTGTTACGTTTTGTTGT2051911.453978106875514No Hit
AAAGATAATAAATTGGGTATTGTTGAAATGTAATTAATTAAATAGTTTTT1833110.232607470009993No Hit
GGAATTGTTTTTGGGAATTTGTAGCGTTGGTTTCGTTAAGTCGGTTGGAA170149.497440592152637No Hit
GTGTTTGGAGTGTTGTTTTTTTAGGGATGGTGTTTGTAGTGATAGGGTTG165869.258525312180772No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTCGAGTTTGGTTTTTTTTTATT129837.247282896903591No Hit
TGGGGAGGGAAGAGTAGATTTTACGATATTTTTGGGGTTTTTAGATAGTT104695.843934733704359No Hit
GTTTTATTGGGTTAATGAGAAGGGAGGTCGGTAGGGTTGAGCGTTAGGTT82344.59632807310361No Hit
TAGATGTAATAGGTTTTTTTGGGGGGATTTTTAGGGAGTGGTTGGGTAGA56673.163394606543376No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTCGAGCTTGGTTTTTTTTTATT46532.5973663497876No Hit
ATTATTTTTTTAGGGAGTTGGGTTGTATTTTATTTGTTGTTTTGTTTTTA40872.2814176384229357No Hit
GTTTTATTGGGTTAATGAGAAGGGAGGTTGGTAGGGTTGAGTGTTAGGTT28941.6154692061649074No Hit
GTTTTATTGGGTTAATGAGAAGGGAGGTCGGTAGGGTTGAGTGTTAGGTT27301.5239222297270896No Hit
TGGGGAGGGAAGAGTAGATTTTACGATATCTTTGGGGTTTTTAGATAGCT19341.0795844660410958No Hit
TAGATGTAATAGGTTTTTTTGGGGGGATTTTTAGGGAGTGCTTGGGTAGA18031.006458527545034No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTCGAGTTTGGTTTTTTTTATTT15110.8434602524240411No Hit
GTTTTATTGGGTTAATGAGAAGGGAGGTCGGTAGGGTTGAGCGTTGGGTT12500.6977665887028799No Hit
GTTTTATTGGGTTAATGAGAAGGGAGGTTGGTAGGGTTGAGCGTTAGGTT9690.5409086595624725No Hit
AAAGATAATAAATTGGGTATTGTTGAAATGTAATTAATTAAATAGTTTTC9320.5202547685368671No Hit
GGGAGATTAAGTAGGTGAGTGGTTTGTTTCGAGTTTGGTTTTTTTTTATT8930.4984844509693374No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTCGAGTTTGGTTTTTTTTTTAT8400.46889914760833523No Hit
ATTATTTTTTTAGGGAGTTGGGTTGTATTTCATTTGTTGTTTTGTTTTTA8330.4649916547115991No Hit
GTGTTTGGAGTGTTGTTTTTTTAGGGATGGTGTTTGTAGTGATAGAGTTG5810.3243219104290985No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTCGAGCTTGGTTTTTTTTATTT5280.29473660706809646No Hit
GTGTTTGGAGTGTTGTTTTTTAGGGATGGTGTTTGTAGTGATAGGGTTGG2660.14848473007597282No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTCGAGCTTGGTTTTTTTTTTAT2600.14513545045019902No Hit
GTTTTATTGGGTTAATGAGAAGGGCGGTCGGTAGGGTTGAGCGTTAGGTT2150.12001585325689533No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)