FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888233

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888233
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179143
Sequences flagged as poor quality0
Sequence length151
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT2133711.910596562522677No Hit
AAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC175459.793851839033621No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA175379.789386132865923No Hit
CACACACACAAAACTATAATAAATACCCTAAAAAACACTCCGTAAAATTC164089.15916334994948No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA148078.265463903138834No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACAAAAAACCCAAATATCCT91845.126630680517798No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA58113.243777317561948No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT39662.213873832636497No Hit
ACTCAAACTAATACACCACTACATACCGCCCCCTAAAACCCCCTCCTTCC37232.0782280077926574No Hit
CTATTAACCCCCTCCCTAAACAATCACCATTTATATTATTTATTTCCTAA34851.945373249303629No Hit
ACTCAAACTAATACACCACTACATACCACCCCCTAAAACCCCCTCCTTCC30961.7282282868992926No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAATA19801.1052622765053615No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT17720.9891539161452025No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAATA14630.8166660154178506No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAA11970.6681812853418777No Hit
CCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA10020.5593296975042285No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAA9090.5074158633047342No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAATAA8210.4582930954600515No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAGAAT7560.4220092328475017No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACAAAAAACCCAAATATCCT7200.40191355509285875No Hit
ACCCCACTCCAACTATCCAAAAAAAAACTCTAAAAATAAAAAAAAAATAA5440.3036680194034933No Hit
CAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC5330.297527673422908No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAGAATA4840.27017522314575504No Hit
AAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAGTAATAC4120.2299838676364692No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAAAT4060.2266345880106954No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAT4020.22440173492684615No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACTTCTAAAA3460.19314179175295715No Hit
CCCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT3450.19258357848199484No Hit
CCAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTTA3060.170813260914465No Hit
CTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAAA2860.1596489954952189No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAAACTCTAAAAATAAAAAAAAA2830.15797435568233198No Hit
CCAAAAAAACCAACAAAAAACTAAATAAAACCGAAAACTTCTCCATCTTA2830.15797435568233198No Hit
CCTCAAACTAATACACCACTACATACCGCCCCCTAAAACCCCCTCCTTCC2810.1568579291404074No Hit
GAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC2740.15295043624367125No Hit
CCTCAAACTAATACACCACTACATACCACCCCCTAAAACCCCCTCCTTCC2490.13899510446961366No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAATTAAACAAAACGTCTAAAAA2460.13732046465672673No Hit
CAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA2460.13732046465672673No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAGAA2380.1328547584890283No Hit
CACACACACAAAACTATAATAAATACCCTAAAAAGCACTCCGTAAAATTC2230.12448155942459377No Hit
CACACACACAAAACTATAATAAATACCCTAAAAACACTCCGTAAAATTCA2100.11722478690208381No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAAATTAAACAAAACGTCTAAA2050.11443372054727229No Hit
TCTCTTTACATTTAATCAAAACCCAAAAAATTAAACAAAACGTCTAAAAA1950.10885158783764926No Hit
GCCAAACAACCAAACTAAACAACGCTAAAAACAAAAAACCCAAATATCCT1850.10326945512802621No Hit
ACCCCACTCCAACTATCCAAAAAAAAACTCTAAAAATAAAAAAAAATAAA1820.10159481531513931No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)