FastQCFastQC Report
Fri 2 Sep 2022
EGAF00000888235

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888235
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences176434
Sequences flagged as poor quality0
Sequence length151
%GC30

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTT2127112.056066291077682No Hit
CACACACACAAAACTATAATAAATACCCTAAAAAACACTCCGTAAAATTC1873710.619835179160479No Hit
AAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC152818.661029053357062No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA137357.784780711200789No Hit
ACCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT135277.666889601777435No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCTAA122096.919868052642915No Hit
AAAAACTAACACACACTTATACCCCCGAAACAACCATAAATACACAAAAA51682.9291406418264057No Hit
CCAAAAAAACCAACAAAAAAACTAAATAAAACCAAAAACTTCTCCATCTT46622.6423478467869006No Hit
CTATTAACCCCCTCCCTAAACAATCACCATTTATATTATTTATTTCCTAA43302.4541754990534703No Hit
ACTCAAACTAATACACCACTACATACCGCCCCCTAAAACCCCCTCCTTCC42792.425269505877552No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT41322.341952231429316No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAATA25581.4498339322352833No Hit
ACTCAAACTAATACACCACTACATACCACCCCCTAAAACCCCCTCCTTCC22591.2803654624392125No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAATA16170.9164900189305917No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAA13510.7657254270718796No Hit
CCCAAACAACCAAACTAAACAACGCTAAAAACGAAAAACCCAAATATCCT9910.5616831223006904No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACGTCTAAAA9810.5560152805014906No Hit
CTATTAACCCCCTCCCTAAACAATCACCGTTTATATTATTTATTTCCCAA9800.5554484963215707No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAATAA9330.528809639865332No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAA8530.4834669054717345No Hit
CCTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAA8160.46249589081469555No Hit
ACCCCACTCCAACTATCCAAAAAAAAACTCTAAAAATAAAAAAAAAATAA6100.34573834975118173No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAACTCTAAAAATAAAAAAAAAT4060.23011437704750787No Hit
ACCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAAAT3930.22274618270854826No Hit
CAATTACAAAAATAAACTAAAATCCCAACACATCAAAAATACAATAATAC3870.21934547762902842No Hit
ACCCCACTCCAACTATCCAAAAAAAAAAAAACTCTAAAAATAAAAAAAAA3400.19270662117278983No Hit
CCCCCACTCCAACTATCCAAAAAAAAAAACTCTAAAAATAAAAAAAAAAT3350.18987270027318998No Hit
CCAAAAAACCAACAAAAAAACTAAATAAAACCGAAAACTTCTCCATCTTA3030.17173560651575093No Hit
CCTCAAACTAATACACCACTACATACCGCCCCCTAAAACCCCCTCCTTCC2750.15586564947799175No Hit
CCAAAAAAACCAACAAAAAACTAAATAAAACCGAAAACTTCTCCATCTTA2750.15586564947799175No Hit
ACCCCACTCCAACTATCCAAAAAAAAACTCTAAAAATAAAAAAAAATAAA2360.13376106646111294No Hit
CTCTTTACATTTAATCAAAAACCCAAAAAATTAAACAAAACATCTAAAAA2260.12809322466191322No Hit
CACACACACAAAACTATAATAAATACCCTAAAAACACTCCGTAAAATTCA2110.1195914619631137No Hit
TCTCTTTACATTTAATCAAAAACCCAAAAAAATTAAACAAAACATCTAAA2000.11335683598399401No Hit
CCCCCACTCCAACTATCCAAAAAAAAAACTCTAAAAATAAAAAAAAAATA1910.10825577836471428No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)