FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888241

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888241
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences330359
Sequences flagged as poor quality0
Sequence length301
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT120763.655417288464973No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT104033.1489985137380847No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT88962.692828105182544No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT83242.5196831325921196No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC76362.3114248438819587No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC74522.255727859692032No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA73952.238473902633196No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG70432.131923150269858No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA67342.038388540950905No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA65221.9742159287320764No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA64061.9391026126123398No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC61331.8564652393305465No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC60901.8434490962861614No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC58171.7608117230043678No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA57651.7450712709506928No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT53751.627017880548131No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT53311.6136990365027137No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC52661.59402347143562No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC50641.5328778692271137No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA49841.5086617891445366No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA45181.3676031226635266No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC44041.3330952085458547No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC40861.2368362902176118No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA40091.2135283131381316No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA40001.2108040041288415No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC36141.0939614177304084No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA35431.0724696466571215No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC33691.019799672477517No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA32860.9946754893918435No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC32600.986805263365006No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT29830.9029570860790836No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCGAAACCTTATT29270.88600583002128No Hit
AAACTACAAAATCAAAACCAACCCAAAACCGCGCCAAACCCCCACAAAAC27460.8312169488344499No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA25530.7727956556352331No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAACAACAT22090.6686665112801529No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT21640.6550449662337033No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA21550.6523206572244135No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT21240.6429369261924149No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC21000.6356721021676418No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA20050.6069155070695819No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC19360.5860291379983593No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC17740.5369915758311413No Hit
ACTCCCACACACACTAACACAATAAAACGACGCTACGAACATCAATACAC17500.5297267518063682No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC16930.5124727947475322No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC16600.5024836617134693No Hit
AAACTACAAAATCAAAACCAACCCGAAACCACACCAAACCCCCGCAAAAC15660.47402976761644156No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACGAAAAAAATAATCACA15460.4679757475957973No Hit
TCCAACCAATAACACTATCTCTAACCTCAATTTCCTCATCTATCCTATAA15380.4655541395875396No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCAAACATTAAAACCTTCATC14200.42983542146573883No Hit
CCCAAAAATTAATAAATAAACCCCAAAAACTAAAAAATAAACACCAAAAA13120.3971437133542601No Hit
AAAAAAATCCTTCCAACCAATAAAAACGACAACTAACCAAATAAATAACT12620.38200866330264954No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAACTTAACTATATC11940.36142499523245925No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT11620.35173856319942853No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA10220.309360423054919No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCGCAA9970.3017928980291138No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA9650.29210646599608303No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA9510.28786865198163214No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA8880.26879848891660285No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC8480.25669044887531445No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA7910.23943649181647841No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAACAACATT6940.21007449471635403No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT6240.1888854246440993No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA6060.18343680662551953No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA5960.1804097966151974No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAACAACACAA5670.1716314675852633No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAACAAAAACACCTAACGCTAA5640.1707233645821667No Hit
AATCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC5250.15891802554191045No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCGCCAAAAAAACCC4980.15074509851404078No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC4970.15044239751300859No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAAACAACA4720.14287487248720332No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA4460.13500464646036583No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAGCCCCCACCACCCACAA4140.12531821442733512No Hit
CATAATCACCCACAACAAATTTCCCATCACCAAAATAAACCACCCCCTAA3950.11956689540772311No Hit
CAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT3890.11775068940152984No Hit
CCTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC3890.11775068940152984No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAAACAAAAACACCTAACGCT3870.11714528739946543No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT3670.11109126737882122No Hit
CCCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA3600.10897236037159576No Hit
CCTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT3590.10866965937056353No Hit
CACTCCATTATTCTTATCTTTAATCTCTTTTTATTTATAAAAATCCAATT3540.10715615436540249No Hit
CCATAAAAACAAAAACCTAAAAAAACTAAAACACCTATTAACCTATCCCG3460.1047345463571448No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)