FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888243

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888243
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences290066
Sequences flagged as poor quality0
Sequence length301
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT80202.764887991008943No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT78062.691111678031896No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC65982.27465473375025No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA65642.262933263464177No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA61872.132962842939193No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT59402.0478098088021346No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC59252.0426385719112203No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT53881.857508291216482No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC53771.8537160508298112No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT53491.844063075300104No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC52891.8233781277364463No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA52471.8088986644418856No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC48651.677204498286597No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA47851.6496245682017197No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC47641.6423848365544396No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA47451.6358346031592812No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA46941.6182523977301715No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT46841.6148049064695622No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC46461.6017044396792455No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT43301.4927637158439804No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC42931.4800079981797247No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA42141.4527728172209085No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC40991.4131266677238974No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA40491.395889211420849No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC38251.318665407183193No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA36401.2548868188619142No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA33191.1442223493963444No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC30261.0432108554604813No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG28860.9949459778119462No Hit
ACTCCCACACACACTAACACAATAAAACGACGCTACGAACATCAATACAC28250.9739162811222274No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC27940.9632290582143375No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA27040.9322016368688506No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC26670.9194459192045948No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA25180.8680782994215109No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA24800.8549778326311942No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT22290.7684458019898919No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT19230.6629525694152366No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACACT17500.6033109706066895No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC15040.518502685595692No Hit
TACCACCTAAATCTCCAACTTCTCCGAAAAAACCAACTATCCTAATAAAA14470.49885198541021697No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACAAAAAAAATAATCACA13840.47713279046837614No Hit
CCCAAAAATTAATAAATAAACCCCAAAAACTAAAAAATAAACGCCAAAAA13250.4567925920307792No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAACAACAT13070.4505871077616818No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC12860.4433473761144015No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC12720.43852088834954805No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC12610.4347286479628774No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCGCAA12430.4285231636937801No Hit
CTCATTCAAAAAACCCAAAAAACCGAACCCAACTCGTTCCGCGCCAACAT12240.4219729302986217No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC11020.3799135369191839No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA10820.3730185543979646No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT10510.3623313314900747No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA9110.31406645384153953No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA9020.31096371170699083No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA9020.31096371170699083No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATGA8900.3068267221942592No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC8340.2875207711348452No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCACATCA7980.2751098025966504No Hit
AAACTACAAAATCAAAACCAACCCGAAACCACGCCAAACCCCCACAAAAC7560.2606303393020899No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCGAAAAAAAAACAACACAA7030.24235863562085871No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATCAATTTATAC6300.21719194941840822No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT6300.21719194941840822No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT6210.21408920728385955No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCGAACATTAAAACCTTCATC6050.20857322126688407No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCCACCTCCGCCT5600.19305951059414064No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAACAAAAACACCTAACGCTAA5350.1844407824426165No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCGCCAAAAAAACCC5110.17616680341715332No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC5100.17582205429109238No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA4770.1644453331310805No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAACTTAACTATATC4670.16099784187047086No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCACAA4410.15203436459288577No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAACAACATT4370.15065536808864188No Hit
ACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC4300.14824212420621513No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA4230.14582888032378838No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCGAACCCTAAATCCCACC4000.13789965042438618No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA3650.1258334310122524No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA3620.12479918363406947No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAAACAAAAACACCTAACGCT3530.1216964414995208No Hit
CCTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT3500.12066219412133791No Hit
CCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA3480.11997269586921597No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAC3150.10859597470920411No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCACAC3080.10618273082677734No Hit
TAATCTAAACCTCCTAACCTCAAACAATCTATCTACTTCTACCTCCACCT3040.1048037343225335No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACTCAACAACCTAAACCCTGTA3020.10411423607041155No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACGAA2970.10239049044010674No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)