FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888245

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888245
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences347347
Sequences flagged as poor quality0
Sequence length301
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT106973.0796293044131664No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT89912.5884778046161334No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC87382.5156399796169246No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA81572.348372089006095No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC80352.3132487109432356No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT77482.2306224035330664No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC73372.1122969249770405No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT72442.0855225466176472No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC72002.072855098791698No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA71552.0598997544242503No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC68951.98504665363455No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC68371.9683486542276167No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA63351.8238245903951955No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA62291.7933075569963177No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA55131.5871736332831434No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC54751.5762335647061871No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG54561.570763530417709No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT54201.5603992549237506No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT53661.544852841682813No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC53441.538519117769838No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC52191.5025320500824824No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA50691.4593475688576554No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA44251.2739421961323978No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA43791.2606989552234509No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCGAAACCTTATT39381.1337365804224593No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA39091.1253875807189928No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA37931.0919915819051267No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA37661.0842183752846577No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC37491.0793241340791773No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT37171.0701114447512141No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC30750.8852818651089546No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC30600.8809634169864718No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA30230.8703112449510143No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAGTAA28020.8066861092797692No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT27690.7971855234103072No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT27190.7827906963353649No Hit
AATATTTAAAAAACCCTACCTTAAAAACGCATTCACTTACCAATCCACTC24540.7064981128381705No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACACACACTTCAAT21720.6253112881354956No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA20940.6028553578985856No Hit
TACCACCTAAATCTCCAACTTCTCCGAAAAAACCAACTATCCTAATAAAA18270.5259869813183935No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC17210.49546994791951565No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA16380.47157453497511137No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC14750.4246473987107993No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC13840.39844881343440425No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCGCAA13800.39729722726840877No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC13140.3782960555294849No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT12480.359294883790561No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA12070.3474911255891083No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC11850.3411574016761337No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT11810.34000581551013825No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT11150.32100464377121435No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCGCTTACCAATCCACTC10860.3126556440677478No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC10370.2985487135343043No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC9890.2847296795423596No Hit
CCCAAAAATTAATAAATAAACCCCAAAAACTAAAAAATAAACACCAAAAA9550.27494119713139886No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA9520.2740775075069023No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC8700.25046999110399687No Hit
CAACCATAAATACACAAAAAACAACCTATGCCACTGCTGGATACACGACC8570.24672733606451186No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA8450.2432725775665257No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACAAAAAAAATAATCACA8050.2317567159065718No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA8010.23060512974057645No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCGAACCCTAAATCCCACC7840.22571088853509605No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA7780.22398350928610294No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACAAAAAAAATAATCACA7610.21908926808062254No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAACAACAT7580.21822557845612603No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAACTTAACTATATC6620.19058751047223668No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA6290.18108692460277476No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCACAC6290.18108692460277476No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCGCCAAAAAAACCC5600.1612220632393543No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAACAAAAACACCTAACGCTAA5470.1574794081998693No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA5390.15517623586787851No Hit
CCTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC5360.15431254624338198No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA5100.14682723616441196No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA4970.14308458112492695No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCGCAAAAC4920.14164509841743272No Hit
CCCAAAAATTAATAAATAAACCCCAAAAACTAAAAAATAAACGCCAAAAA4630.13329609871396614No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACGCCTCCAT4620.1330082021724673No Hit
CCATAAAAACAAAAACCTAAAAAAACTAAAACACCTATTAACCTATCCCG4360.12552289209349726No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAACAACACAA3890.11199175464305147No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAACAACATT3590.10335485839808606No Hit
CCTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT3580.10306696185658723No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAAACAAAAACACCTAACGCT3550.10220327223209068No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)