FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences255048
Sequences flagged as poor quality0
Sequence length301
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGGGTAGATGATTTTTTTATGGTTTTAGGTTTCGGAAGTATGGTATTT216068.4713465700574No Hit
GGAAAGTTAGGTAGTGAGTTGTTTGATATTTGTAGTATTTATGTTGGAGT166106.512499607916941No Hit
AGAAGGGTTAGTTATAGGGGAGGATGTGGTATAGGTGTTTATGGGATTTT154086.04121577114896No Hit
GAAAGGGTTTTTTGAGAAGGTTATTTTGTTTGGAGTGGGGGCGTCGGGTA139205.457796179542675No Hit
GGAAATTTGAATTTTTATTTGGTATTTTTTTTTAGTTTTATTTTTAAATT108194.241946613970703No Hit
GTTGGGGATTGAGGTTTTTAGAGTAGAGGAGTTAGTTGTGGGTATTAGTG100293.9322009974593017No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGTTCGTTATTTTACGATGAGTTAG99893.916517675104294No Hit
GGGTTTAGGTTGTTGAGTTTTGGCGGGAAGGGTATTTTTTTTCGTTTATT91113.5722687494118754No Hit
GGAGTTGAGTGGTATAGTTGGTATTATTACGTATTAGTAGAGGTTTTTTG89673.5158087889338474No Hit
TGGTTTTTATTTTTGTGTTTTTTGGGGATGGTTTTTAAGGATAGTATGTT87583.4338634296289325No Hit
GGTTATTTTAGTTATTTTTGGTTAATTTAGTATTTTTGATTTTTTTGATT84923.3295693359681313No Hit
GATTGTTTAGGGAGGGGGTTAATAGCGTGTTAGTATTGAGGTTAGGTTTG79113.1017690787616448No Hit
TTAGGTAGATTTTGGAATTTGTGTTTTCGGTGATTGTTTCGGAGGAGAGG69962.743013079890844No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTGT64252.5191336532731095No Hit
AATTTGTTGTGGGTGATTATGGTGTATGGAGTTAGTGTGGATTTGTGTGG48481.9008186694269316No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTTAATTAATTGAGAAGTTGT48061.8843511809541733No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTAATTAATTGAGAAGTTGTT43341.6992879771650826No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTGTT42461.6647846679840657No Hit
GGGTTTAGGTTGTTGAGTTTTGGCGGGAAGGGTATTTTTTTTCGTTTATC28691.1248862959129262No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTAATTAATTGAGAAGTTGTTT21490.8425864935227879No Hit
GGAAATTTGAATTTTTATTTGGTATTTTTTTTTTAGTTTTATTTTTAAAT20460.8022019384586431No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTTG18230.7147674163294753No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTTTAATTAATTGAGAAGTTG16780.6579153727925724No Hit
GGAAATTTGAATTTTTATTTGGTATTTTTTTTAGTTTTATTTTTAAATTT15330.6010633292556695No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTGTTT13310.5218625513628807No Hit
GGAGTTGAGTGGTATAGTTGGTATTATTATGTATTAGTGGAGGTTTTTTG10360.4061980489946991No Hit
TAGTTTTGGGGTTTTTAGGATTTTTCGGGTTGTTGTTTTTGTTTTTGTTT9810.3846334807565635No Hit
AGGTATTAGGGAGTTGTATGGGGTTGGTTTTGATTTTGTAGTTTAGACCA9440.3701264075781814No Hit
TTTTTTTTGGGTTTTTAGGGTAGAGTTCGGGTTGGGGAGGTTAGTGTTTT8580.33640726451491487No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTTT7820.3066089520404003No Hit
TTTTTTTTGGGTTTTTAGGGTAGAGTTCGGGTTGGGGAGGTTAGTGTTTC7630.2991593739217716No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTAATTAATTGAGAAGTTGTTTT7480.29327812803864367No Hit
GAAGTTTTGGGTGAAGTTAATTTTTATTTATTATTTTTGTTTTTTTTAAT7480.29327812803864367No Hit
GTTTATGAGTAGGTTTTTTTGGGGTTTAGTTTTAAGACCAAGTCTCTGCT6930.27171355980050815No Hit
TGAGGGTAGATGATTCTTTTATGGTTTTAGGTTTCGGAAGTATGGTATTT5620.22035067908785796No Hit
GGGTTTAGGTTGTTGAGTTTTGGCGGGAAGGGTATTTTTTTCGTTTATTG5420.21250901791035412No Hit
TGAGGGTAGATGATTTTTTTATGGTTGTATGTGTTAGTAATGAAGATTAT5180.20309902449734954No Hit
GGGTTTAGGTTGTTGAGTTTTGGCGGGAAGGGTATCTTTTTTCGTTTATT4310.1689877983752078No Hit
TAGTTTTGGGGTTTTTAGGATTTTTTAGGTTTTTTAGATAGGGCGGGTTT4080.1599698880210784No Hit
TGAGGGTAGATGATTTTTTATGGTTTTAGGTTTCGGAAGTATGGTATTTG3910.15330447602020014No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTTTTAATTAATTGAGAAGTT3900.15291239296132492No Hit
GGGAGATTAAGTAGGTGAGTGGTTTATTTATTAATTTTTGGGAGACCAAG3720.14585489790157147No Hit
GGAAATTTGAATTTTCATTTGGTATTTTTTTTTAGTTTTATTTTTAAATT2660.1042940936608011No Hit
AAGGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTGTTTT2660.1042940936608011No Hit
GTTGGGGATTGAGGTCTTTAGAGTAGAGGAGTTAGTTGTGGGTATTAGTG2570.10076534613092437No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)