FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888249

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888249
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences255048
Sequences flagged as poor quality0
Sequence length301
%GC31

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT205748.066716853298203No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC149325.854584235124369No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT143895.641683134155139No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT139585.472695335779932No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA135615.31703836140648No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC135045.2946896270505945No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA111204.359963614692136No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG93933.6828361720146794No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA89993.5283554468178537No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA89323.502085881873216No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCAACCTTAAAAAACAATACC86863.4056334493899185No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC80773.1668548665349268No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA67982.665380634233556No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA64422.5257990652739877No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC45111.7686866785859918No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAGAACAAATCCCGCATCG35271.3828769486528025No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC24070.9437439227125874No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC16990.6661491170289514No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCGCCAAAAAAACCC10460.410118879583451No Hit
CCTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC10350.40580596593582385No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA10070.3948276402873185No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAGAAATACTAAA7930.3109218656880274No Hit
CCCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA6880.2697531445061322No Hit
CAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT6870.269361061447257No Hit
CCTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA6730.2638718986230043No Hit
TAAAACTAAACCCCACATACCTTCCTAGCAGAGACTTGGTCTTAAAACTA6470.25367773909224933No Hit
TACCACCTAAATCTCCAACTTCTCCCCCCCAACCCCATCAAAAAAAAAAA6000.2352498353251153No Hit
ACTCCCACACACACTAACACAATAAACAACGCTACGAACATCAATACACT5660.22191901132335873No Hit
CAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA4830.18937611743671778No Hit
CTAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA4730.1854552868479659No Hit
TAATCTAAACCTCCTAACCTCAAACTATCTATCTACTTCTACCTCCGCCT4330.16977196449295817No Hit
TACCACCTAAATCTCCAACTTCTCCTATCAACCCACCCAAAACCTTTACC4290.16820363225745744No Hit
CTACAAAACAAACCCCTATCTCCTCTACCGCAACCTTAAAAAACAATACC4290.16820363225745744No Hit
TAATCTAAACCTCCTAACCTCAAACAATCCACCTACCTCAACCTAATTAA4100.16075405413882876No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCACCAAAAAAACC4050.1587936388444528No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAACCCAATTCACCCAT3700.1450707317838211No Hit
CATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT3340.13095574166431417No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACA3290.1289953263699382No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCACCAAAAAAACCC3230.12664282801668705No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACAGCCAAAAAAACC2670.1046861767196763No Hit
CACTCCATTATTCTTATCTTTAATCTCTTTTTATTTATAAAAATCCAATT2580.10115742918979957No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)