FastQCFastQC Report
Sat 3 Sep 2022
EGAF00000888250

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888250
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences223564
Sequences flagged as poor quality0
Sequence length301
%GC34

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAAATATTGTTGTTTTTTAAGGTTTGTGTGTTATTTTGTGATGGGTTAG174707.8143171530300055No Hit
GAGTATGTGGTTTTTTTATGTTGGGTTTTTTTTTATGGGATAGGGAGTAT164737.368359843266357No Hit
GTTGGGGATTGAGGTTTTTAGAGTAGAGGAGTTAGTTGTGGGTATTAGTG161687.231933585013688No Hit
AGGAAGGTAGTTAAGAGGGGTATTTTTGTTTTTTGTTTGAGGTTTTTTGT149976.70814621316491No Hit
TGATTTTGTGAGTTTATAGGTTTAGGTTTTAGTAGTAGAATTATTTTGGT132225.914190120055107No Hit
GGTTATTTTAGTTATTTTTGGTTAATTTAGTATTTTTGATTTTTTTGATT127015.681147233007103No Hit
GTTTATGAGTAGGTTTTTTTGGGGGTTGTGTAGAGGGTTCGTTGGGGTTT123225.511620833407884No Hit
TGGTTTTTATTTTTGTGTTTTTTGGGGATGGTTTTTAAGGATAGTATGTT116755.222218246229268No Hit
GTGAAGTTTTTGGTTTTTTGTTGTATAGATGAGGTTGGGATGTAAGGAGA116295.20164248268952No Hit
AGGTATTAGGGAGTTGTATGGGGTAGTGTTTGGGTTGTGGTGGGGGGTAT104694.682775402121988No Hit
GTAGGTTTTTGGTTTTAGATTGGGTTTTGATGTTGAGTGTGAAGAAGTGG82073.6709845950152973No Hit
GAGTATGTGGTTTTTTTATGTTGGGTTTTTTTTATGGGATAGGGAGTATT42551.903258127426598No Hit
GTAGGTTTTTGGTTTTAGATTGGGTTTTGATGTTGAGCGTGAAGAAGTGG41491.855844411443703No Hit
GTTTATGAGTAGGTTTTTTTGGGGTTGTTGTATTGTGTTATGGATGTTTT26131.1687928288991072No Hit
AGGTATTAGGGAGTTGTATGGGGTTGGTTTTGATTTTGTAGTTTAGACCA20520.9178579735556709No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTCGAGTTTGGTTTTTTTTTATT19550.8744699504392479No Hit
GAAGTTTTGGGTGAAGTTAATTTTTATTTATTATTTTTGTTTTTTTTAAT14030.6275607879622838No Hit
GAGTATGTGGTTTTTTTATGTTGGGTTTTTTTTTTATGGGATAGGGAGTA13110.5864092608827898No Hit
GGGAGATTAAGTAGGTGAGTGGTTTATTTATTAATTTTTGGGAGACCAAG9080.40614768030631054No Hit
TGATTTTGTGAGTTTATAGGTTTAGGTTAGAGATAGTGTTATTGGTTGGA7730.3457622873092269No Hit
GTTTATGAGTAGGTTTTTTTGGGGAAGATTAGGGGTTGGTAGAGTTTTTA6430.2876133903490723No Hit
GGAAATATTGTTGTTCTTTAAGGTTTGTGTGTTATTTTGTGATGGGTTAG4750.21246712350825714No Hit
GAGTATGTGGTTTTTCTATGTTGGGTTTTTTTTTATGGGATAGGGAGTAT4370.19546975362759655No Hit
GTTGGGGATTGAGGTCTTTAGAGTAGAGGAGTTAGTTGTGGGTATTAGTG4090.18294537582079404No Hit
TTGTGTGATGGGGATAGTTTATTTTTTATTTTTTGAGTTTTTATATTTAT3610.16147501386627544No Hit
GAGTATGTGGTTTTTTATGTTGGGTTTTTTTTTATGGGATAGGGAGTATT3430.15342362813333096No Hit
GTGAAGTTTTTGGTTCTTTGTTGTATAGATGAGGTTGGGATGTAAGGAGA3340.1493979352668587No Hit
GAGTATGTGGTTTTTTTATGTTGGGTTTTTTTATGGGATAGGGAGTATTT3260.14581954160777227No Hit
GTTTATGAGTAGGTTCTTTTGGGGGTTGTGTAGAGGGTTCGTTGGGGTTT3190.14268844715607165No Hit
GGAAATATTGTTGTTTTTAAGGTTTGTGTGTTATTTTGTGATGGGTTAGA3140.1404519511191426No Hit
GGTTATTTTAGTTATCTTTGGTTAATTTAGTATTTTTGATTTTTTTGATT3000.13418976221574136No Hit
TGGTTTTTATTTTTGCGTTTTTTGGGGATGGTTTTTAAGGATAGTATGTT2870.1283748725197259No Hit
TAGGTTGTTTTGGTTTAGAGAGGGCGGGAGTTTTTGTAGGATAATATAGT2810.12569107727541107No Hit
GTTGGTTTTTTTGGGGTGAAGTTGGTATATGTTTTTAAAGGATAGTGAAA2790.12479647886063946No Hit
AGGAAGGTAGTTAAGAGGGGTATTTTTGTTTTTTGTTTGAGGTTTTTGTA2590.11585049471292336No Hit
GGGAGATTAAGTAGGTGAGTGGTTCGTTTCGAGTTTGGTTTTTTTTATTT2540.11361399867599435No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2530.11316669946860854No Hit
GAGTATGTGGTTTTTTTATGTTGGGTTTTTTTTTATGGGATAGGGAATAT2470.11048290422429372No Hit
GTAGGTTTTTGGTTTTAGATTGGGTTTTGATGTTGAGTGGGAAGAAGTGG2430.10869370739475048No Hit
GTTGGGGATTGAGGTTTTTAGGGTAGAGGAGTTAGTTGTGGGTATTAGTG2330.10422071532089246No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)