FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888253
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences275924
Sequences flagged as poor quality0
Sequence length301
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT65562.37601658427683No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT64802.3484727678636146No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC62152.2524318290543777No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA61092.2140154535306826No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC59862.1694379611777155No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT59052.1400820515794203No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA58572.122685957002653No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC56352.042229019585103No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT55202.0005508763282642No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA53381.9345906843913543No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA51661.8722546788246037No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC47731.7298241544773199No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA46951.7015555007900727No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC45771.658790101622186No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT45461.6475551238746902No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT43421.573621721923428No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA43131.5631115814499645No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA42661.546077905510213No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC42101.5257824618373175No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC39341.4257549180209044No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT38681.4018352879778488No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA37111.3449355619663386No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC36091.3079688609907074No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA35721.2945593714211159No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC34771.2601296009045968No Hit
CCATAAAAACAAAAACCTAAAAAAACCTATGTAGAACCATGTCGTCAGTG34581.253243646801293No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC34581.253243646801293No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC32491.1774981516649512No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA31951.1579275452660878No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG31391.1376321015931923No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC27390.9926646467867963No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA26850.973094040387933No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA25710.9317783157681101No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT25270.9158318957394065No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC24890.9020599875327989No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA24500.8879256606891752No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC23940.86763021701628No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCAAACATTAAAACCTTCATC20770.752743509082211No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT20710.7505689972601151No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC17950.650541453443702No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCGATTCACCCA15180.5501514909902727No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCGCAA14240.5160841391107696No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAACAACAT14080.5102854409185138No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC14060.5095606036444819No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAACTTAACTATATC13790.49977530044505003No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA13190.4780301822240907No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAC12430.4504863658108755No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA11860.429828503500964No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCACAA11410.41351966483524444No Hit
CCCAAAAATTAATAAATAAACCCCAAAAACTAAAAAATAAACGCCAAAAA10950.39684840753250894No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC10640.3856134297850133No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA9660.3500964033574463No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACAAAAAAAATAATCACA9040.3276264478624549No Hit
TACCACCTAAATCTCCAACTTCTCCGAAAAAACCAACTATCCTAATAAAA7680.27833751322828026No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACACT7320.26529044229570464No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA7310.2649280236586886No Hit
TAATCTAAACCTCCTAACCTCAAACCTCCTAACCTCAAACCTCCTAACCT7180.26021658137748077No Hit
TAATCTAAACCTCCTAACCTCAAACCTCCTAACCATAAAAAAATCATCTA7140.2587669068294168No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACACTA7100.25731723228135284No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACAACCATCAAACA6980.25296820863716096No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT6900.25006885954103303No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT6820.24716951044490512No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA6490.23520969542337747No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCGAAACCTTATT5480.19860541308476246No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT5430.19679331989968255No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACACACACTTCAAT5410.19606848262565055No Hit
CCTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC4890.17722271350081908No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAACAACACAA4860.1761354575897711No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA4840.17541062031573912No Hit
AAACTACAAAATCAAAACCAACCCGAAACCACACCAAACCCCCACAAAAC4730.17142401530856322No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCAACCTTAAAAAACAATACC4580.16598773575332337No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC4110.14895405981357185No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCACCAAAAAAACC3870.1402560125251881No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACGCCTCCAT3860.1398935938881721No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAACAACATT3810.13808150070309216No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA3760.13626940751801223No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAACAAAAACACCTAACGCTAA3700.13409489569591626No Hit
CCTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA3700.13409489569591626No Hit
ACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC3570.1293834534147084No Hit
CCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA3420.12394717385946855No Hit
AAACACAAACCTATCTCTTATCCCCTACGCCCAAAAAAAAAAACAACACA3220.11669880111914875No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAATACCTAAAATAAA3190.11561154520810077No Hit
CCTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT3010.10908800974181296No Hit
AACCACTCACCTACTTAATCTCCCAATTCCAATATATCCAAAACTTACCC2960.10727591655673302No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)