FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888255

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888255
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences280191
Sequences flagged as poor quality0
Sequence length301
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC93793.3473594797834334No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT82132.9312147784903866No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT78592.8048723906192565No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT78142.7888119175847903No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC68482.4440470964449252No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA65062.321987501382985No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA62232.2209849709662337No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA60692.166022463248284No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA58742.096427080098932No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC56272.0082729281097538No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC52521.874435652822539No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA52101.8594458779903709No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT51901.8523078899750527No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA51721.8458837007612663No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC47161.6831375740120131No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA46791.669932296183675No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA46241.6503028291415498No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC45821.6353130543093817No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA44451.5864178364044528No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC43971.5692866651676893No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC42321.5103982640413147No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT41371.4764928209685537No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC40581.448297768308047No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC39971.4265269048613267No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA39541.411180230628393No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA38501.3740626929487385No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA37321.3319485636583617No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA36931.3180294870284912No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA35461.265565275115903No Hit
ACTCCCACACACACTAACACAATAAAACGACGCTACGAACATCAATACAC31231.1145968285919248No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC30851.1010346513628204No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT30351.0831896813245252No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC27060.965769778472542No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT24990.8918916025139993No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC24540.8758311294795336No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT23090.8240807163684771No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA19240.6866744470736034No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC18420.657408696210799No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCACAA17590.6277860459472289No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAACAACAT16190.577820129840002No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC15860.566042449614727No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC15530.5542647693894522No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA15500.5531940711871545No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC11670.4165016006938124No Hit
ATACAAAACAAACCCCTATCTCCTCTACCACGACCTTAAAAAACAATACC9880.35261660795671523No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCAAACATTAAAACCTTCATC9860.3519028091551834No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA9260.33048884510922905No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT8510.3037213900517861No Hit
ACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC8170.2915868104257453No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT7740.2762401361928113No Hit
AATACCTTAACTCCCTAAAACCCAAAATAACTAAATTAACCAAAAATAAC7430.26517625476906825No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT7290.26017966315834556No Hit
TACCACCTAAATCTCCAACTTCTCCGAAAAAACCAACTATCCTAATAAAA7050.25161407753996384No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAACAACACAA6160.21985003087179816No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAACAAAAACACCTAACGCTAA6120.21842243326873453No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA5730.2045033566388642No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACGAA5360.19129807881052568No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAACAACATT4810.17166861176840084No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA4730.1688134165622736No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAACTTAACTATATC3880.1384769674971716No Hit
CCATAAAAACAAAAACCTAAAAAAACTAAAACACCTATTAACCTATCCCA3690.13169587888261935No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAAACAACA3670.13098208008108755No Hit
CACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC3620.129197583077258No Hit
AATAAAAAACTCCCTCTTCCAAAAATTATTTAAAATTCAAAACCTTATTT3620.129197583077258No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA3510.12527168966883304No Hit
AAAAAAATCCTTCCAACCAATAAAACTAACAATACCTACCCAACATGTAG3470.1238440920657694No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAGACGCACACTTCAAT3340.11920439985581265No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA3330.11884750045504675No Hit
AATCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC3210.11456470764585586No Hit
CAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT3030.10814051843206955No Hit
CCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA2970.10599912202747411No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAAACAAAAACACCTAACGCT2960.10564222262670821No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)