FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888257

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888257
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences334377
Sequences flagged as poor quality0
Sequence length301
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC117153.5035304461730323No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT97222.907496628057552No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT85412.554302478938444No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT84772.5351624065052323No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC79132.366490518187555No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA78542.3488457639131877No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA78232.339574791328351No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC77652.3222291006857527No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC75472.257033228960126No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC73632.2020055207146427No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA69882.0898566588012932No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA60251.8018583814078122No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA59231.7713538909673814No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA57461.7184196281442803No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCGAAACCTTATT56521.6903076467580005No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT54221.6215230114511463No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA53781.6083642116533137No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA53371.5961026027507874No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA53021.5856353756388746No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCGCCCCCTAA51731.5470561671406824No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC47811.4298232234872614No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA47141.409785960158743No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC39811.1905723180721162No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC39541.182497600014355No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA39411.178609772801359No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT38631.1552828095233825No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCGAACATTAAAACCTTCATC38631.1552828095233825No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC37171.1116195192851184No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC37061.10832981933566No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA36951.1050401193862018No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT34401.0287788932851243No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCGCAA27010.8077708694078839No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACACTA26820.8020886604042743No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAACAACAT22450.6713978533212511No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC21780.6513605899927327No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC21470.6420896174078958No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCAACCTTAAAAAACAATACC21000.6280336267147562No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA18180.5436976825559174No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC17310.5176791465920203No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG16900.5054175376894942No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA16810.5027259650035738No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA15950.4770064926714457No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC15540.46474488376891954No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA14970.44769825675809044No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT14900.4456048113357079No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT14470.4327450751696439No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC13890.41539938452704583No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACGAA13260.39655837572560315No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT13100.3917733576173002No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC12570.3759229851335468No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA12170.36396043986278964No Hit
AATACCTTAACTCCCTAAAACCCAAAAATAACTAAATTAACCAAAAATAA11180.3343531403176654No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC10650.318502767833912No Hit
TAATCTAAACCTCCTAACCTCAAACCATAAAAAAATCATCTACCCTCATG10560.31581119514799166No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCAAACATTAAAACCTTCATC10120.30265239535015864No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA9780.292484231870015No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACGACACA9560.2859048319710985No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAACAACATT9430.2820170047581024No Hit
ACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC7340.2195127057183957No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA7280.21771832392778212No Hit
TACCACCTAAATCTCCAACTTCTCCGACTACCTCACCAAAACACTCTCCT6630.19827918786280158No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCGCCAAAAAAACCC6550.1958866788086501No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAACGACACAA6330.18930727890973362No Hit
CCTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC5620.1680737610541395No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAACTCACCCA5020.1501299431480036No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCGCACTCCCTTCTAACACC4890.1462421159350075No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT4850.14504586140793177No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT4770.14265335235378032No Hit
CCATAAAAACAAAAACCTAAAAAAACTAAAACACCTATTAACCTATCCCG4130.1235132799205687No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAACAAAAACACCTAACACTAA3990.11932638907580365No Hit
CCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA3970.11872826181226578No Hit
CCTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA3850.11513949823103863No Hit
TACCACCTAAATCTCCAACTTCTCCCTCTCCCCTCTCAAAAACTCAAACT3810.1139432437039629No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAAACAACA3710.11095260738627358No Hit
ATAATACTCCTTTATCCCAATCCCCCACAAACTAAAACTAAAAAACAAAC3570.10676571654150853No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)