FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888258

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888258
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences247914
Sequences flagged as poor quality0
Sequence length301
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGGGTAGATGATTTTTTTATGGTTTTAGGTTTCGGAAGTATGGTATTT2539910.245084989149463No Hit
TAGGTTGTTTTGGTTTAGAGAGGGCGGGAGTTTTTGTAGGATAATATAGT176967.137959130988972No Hit
AGGAAGGTAGTTAAGAGGGGTATTTTTGTTTTTCGTTTGAGGTTTTTTGT159676.440539864630476No Hit
GGAAATATTGTTGTTTTTTAAGGTTTGTTCGTTATTTTACGATGAGTTAG148155.975862597513654No Hit
GAATTTTGGGTTTGATTTTTGTTTTGGTTCGGGGTGTAGGTGGTGTTGAT142515.748364352154376No Hit
GTTGGGGATTGAGGTTTTTAGAGTAGAGGAGTTAGTTGTGGGTATTAGTG124355.015852271352163No Hit
GATTGTTTAGGGAGGGGGTTAATAGCGTGTTAGTATTGAGGTTAGGTTTG116274.689932799277169No Hit
TGTTGGGTAGGTATTGTTAGTTTTGTTGTATGGATGAAGGCGTGAAGGTT99924.03042990714522No Hit
GGGGTGTATTTATTAGTTTTAGGGATGTATTTTTAGGTTTCGGGGGTTTA78393.1619835910840046No Hit
AGGTATTAGGGAGTTGTATGGGGTAGTGTTTGGGTTGTGGTGGGGGGTAT78183.153512911735521No Hit
TTGTGTGATGGGGATAGTTTATTTTTTATTTTTTGAGTTTTTATATTTAT76203.0736465064498173No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTTAATTAATTGAGAAGTTGT61142.4661777874585544No Hit
AGGTATTAGGGAGTTGTATGGGGTAGTGTTCGGGTTGTGGTGGGGGGTAT60282.431488338698097No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTAATTAATTGAGAAGTTGTT53272.148728994732044No Hit
TTTGGAGAGGAGGTTTTTAGATTTTATTTTGGTGGTATAGCGTTTTTTTT49942.0144082222060877No Hit
TGGGTTGTGATTAGATGGTAGAAAGGGGTTTTAGTTAGGTTGATAATGGT43831.7679517897335368No Hit
GGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTGTTT31381.2657615140734289No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTTTAATTAATTGAGAAGTTG28361.1439450777285671No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTAATTAATTGAGAAGTTGTTT27771.1201465024161603No Hit
GGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTGTTTT21690.8749001669933929No Hit
AGGTATTAGGGAGTTGTATGGGGTTGGTTTTGATTTTGTAGTTTAGACCA15810.637721145235848No Hit
GAAGTTTTGGGTGAAGTTAATTTTTATTTATTATTTTTGTTTTTTTTAAT12280.4953330590446687No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTAATTAATTGAGAAGTTGTTTT10360.4178868478585316No Hit
GGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTTGTT9470.38198730204829096No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTTTTTTTAATTAATTGAGAAGTT9200.37109642860024045No Hit
GGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTGTTTTT7410.298893971296498No Hit
TAGTTTTGGGGTTTTTAGGATTTTTTTTTTTTTTATTTTTTTTAGGTTTT6920.2791290528167026No Hit
TGAGGGTAGATGATTCTTTTATGGTTTTAGGTTTCGGAAGTATGGTATTT6910.2787256871334414No Hit
GTTTATGAGTAGGTTTTTTTGGGGATGGGGAGGATTGGTATAGGGTTGTT6330.2553304775042958No Hit
TAGTTTTGGGGTTTTTAGGATTTTTTTTTTTTTATTTTTTTTAGGTTTTA6160.24847326088885663No Hit
TAGGTTGTTTTGGTTCAGAGAGGGCGGGAGTTTTTGTAGGATAATATAGT4620.18635494566664246No Hit
TGAGGGTAGATGATTTTTTATGGTTTTAGGTTTCGGAAGTATGGTATTTG4080.16457319877054138No Hit
TGTTGGGTAGGTATTGTTAGTTTTATTGGTTGGAAGGATTTTTTTAGACC3800.15327895963922974No Hit
GGAAATATTGTTGTTCTTTAAGGTTTGTTCGTTATTTTACGATGAGTTAG3800.15327895963922974No Hit
GAATTTTGGGTTTGACTTTTGTTTTGGTTCGGGGTGTAGGTGGTGTTGAT3650.14722847439031275No Hit
GTTGGGGATTGAGGTCTTTAGAGTAGAGGAGTTAGTTGTGGGTATTAGTG3090.12463999612768945No Hit
TAGTTTTGGGGTTTTTAGGATTTTTTTTTTTTATTTTTTTTAGGTTTTAT3070.12383326476116717No Hit
TAGTTTTGGGGTTTTTAGGATTTTTTGGGTTGTTGTTTTTGTTTTTGTTT3000.12100970497833927No Hit
TAGTTTTGGGGTTTTTAGGATTTTTTTTTTTTTTTATTTTTTTTAGGTTT2800.11294239131311665No Hit
GGGGATATTTAGGTTGTAAGATAGAGTGGGGTTTTAGACCAAGTCTCTGC2750.11092556289681099No Hit
GAAGGTTTAGGGAATATATTTTTTTTTTAATTAATTGAGAAGTTGTTTTT2750.11092556289681099No Hit
AGGAAGGTAGTTAAGAGGGGTATTTTTGTTTTTCGTTTGAGGTTTTTGTA2720.1097154658470276No Hit
GGGAAAAGGGAGTAGTAAAGTTGTTTTTGTTGAATTTTTTTTTTTTTTGT2640.10648854038093855No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)