FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888263

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888263
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences241579
Sequences flagged as poor quality0
Sequence length301
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC95313.945293258106044No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT72493.000674727521846No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT65602.715467817980868No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC64742.6798686971963623No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC64602.674073491487257No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT64152.655446044565132No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC60652.510565901837494No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA51282.1227010625923612No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC50752.1007620695507474No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA50252.080064906303942No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT48462.005969061880379No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA47121.9505006643789402No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA45711.892134664022949No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC44251.8316989473422771No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC42951.7778863229005835No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT42461.757603102918714No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA42321.7518078972096083No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA42091.742287202116078No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC41411.7141390601004225No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA41401.7137251168354863No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC40921.6938558401185533No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC36791.5228972716999407No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCACCT34181.4148580795516166No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA32681.3527665898112005No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT31611.308474660463037No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA30401.258387525405768No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC29251.2107840499381155No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT28491.1793243618029712No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG26611.101503027994983No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC26581.1002611982001747No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA26471.0957078222858776No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT25461.0538995525273307No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA21680.8974289983814818No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC19600.8113287992747714No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA19290.7984965580617521No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA18700.7740739054305217No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAACAACAT18670.7728320756357133No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA18470.7645532103369912No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACGAA18200.7533767421837163No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACAAAAAAAATAATCACA17360.7186055079290833No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC14430.5973201313028037No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC14320.5927667553885064No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT13420.5555118615442567No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT11200.46361645672844076No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA9420.3899345555698136No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAACTTAACTATACC9250.3828975200658998No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA9160.37917203068147476No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA8890.36799556252819987No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAACAACACAA7470.30921561890727256No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC6260.25912848385000353No Hit
AAAAACCACAAAAACCTTCTCAATATTCATGAAAAAACCCAATTCACCCA6240.25830059732013133No Hit
ATACAAAACAAACCCCTATCTCCTCTACCACGACCTTAAAAAACAATACC6030.24960778875647302No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAACAACATT5960.2467101859019203No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAACAAAAACACCTAACGCTAA4400.18213503657188745No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT4250.17592588759784586No Hit
CCTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC4120.17054462515367644No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA3810.1577123839406571No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAAACAACA3790.1568844974107849No Hit
CCCAAAAATTAATAAATAAACCCCAAAAACTAAAAAATAAACGCCAAAAA3680.1523311214964877No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC3520.14570802925750997No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAAACAAAAACACCTAACGCT3110.12873635539512954No Hit
CCCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA2950.12211326315615181No Hit
CCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA2690.11135073826781303No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCAAACATTAAAACCTTCATC2630.10886707867819637No Hit
CCATAAAAACAAAAACCTAAAAAAACTAAAACACCTATTAACCTATCCCG2570.10638341908857972No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCCAAAAATTTCTAAAAT2550.1055555325587075No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA2460.10183004317428253No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCGCAA2450.10141609990934643No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)