FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888265

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888265
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences297906
Sequences flagged as poor quality0
Sequence length301
%GC31

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT115743.885118124509073No Hit
TAATCTAAACCTCCTAACCTCAAACAATCTATCTACTTCTACCTCCGCCT114093.829731526051842No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT100593.3765684477654023No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA83012.7864494169301723No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC80842.713607648050056No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA80792.711929266278625No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT79862.680711365330003No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC75992.550804616221224No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC73582.4699066148382376No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC72352.4286184232610286No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT71822.4108275764838574No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC69992.3493988036494735No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA69782.342349600209462No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC63682.1375870240948487No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA61852.076158251260465No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT57511.9304747135002316No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA57181.9193973938087854No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT57151.9183903647459266No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC50901.7085926433170195No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC49521.6622693064255167No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA49211.651863339442643No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA48661.633401139956899No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA48521.6287016709968916No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA46061.5461252878424738No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT42101.4131974515451182No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCACAA38311.2859761132706289No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA33091.1107530563332058No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT31651.0624156613159856No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC29360.9855457761844341No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC27880.9358656757500688No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC27430.9207602398071875No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT18610.6246936953267138No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC17380.583405503749505No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC14960.502171826012232No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA14870.49915073882365574No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA14230.4776674521493357No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACAAACATCAATACAC13090.4394003477607031No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA12770.42865870442354304No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCGCAA12610.42328788275496293No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA12450.41791706108638293No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCGCC10730.3601807281491477No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT8780.2947238390633287No Hit
CCCAAAAATTAATAAATAAACCCCAAAAACTAAAAAATAAACGCCAAAAA8040.2698837888461461No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAACAACACAA7770.2608205272804173No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAACAAAAACACCTAACGCTAA7720.25914214550898607No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA6460.2168469248689184No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAGAAACAACAT6050.2030841943431821No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC5490.184286318503152No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACGCCTCCAT5210.17488738058313696No Hit
AAACTACAAAATCAAAACCAACCCGAAACCGCGCCAAACCCCCGCAAAAC4810.1614603264116869No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAAACAAAAACACCTAACGCT4800.16112465005740065No Hit
CCTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC4700.15776788651453816No Hit
ACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC4550.15273274120024435No Hit
AAAAAAATCCTTCCAACCAATAAAACTAACAATACCTACCCAACATGTAG4160.13964136338308059No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAAACAACAC4040.13561324713164555No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC3940.13225648358878303No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT3810.12789269098306177No Hit
CAATCTAAACCTCCTAACCTCAAACAATCTATCTACTTCTACCTCCGCCT3530.11849375306304674No Hit
CCTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA3450.11580834222875672No Hit
CTAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA3070.10305264076587917No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAAATCCTATAACTCTAA3000.1007029062858754No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)