FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888267

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888267
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences277117
Sequences flagged as poor quality0
Sequence length301
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT84883.0629661839584004No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA64632.3322279037374107No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT64522.328258461227568No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC59582.149994406694645No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC57182.0633883882980837No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT56692.045706326208786No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC56282.030911131399373No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA54931.9821952460513068No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA51121.844708191846765No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT50241.8129526517680257No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC49651.7916620055788712No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC48551.7519675804804469No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA45181.630358296315275No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA44201.5949941721366787No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA43301.5625169152379679No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC40631.4661677197717933No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA40341.4557028258822087No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT38441.3871397279849307No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA38291.3817268518351455No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA36211.3066683025581254No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA35941.2969251254885121No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC35471.2799647802191854No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC32561.1749549829133543No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC32381.1684595315336122No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC32351.1673769563036551No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA32281.1648509474337554No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG32001.1547469119541565No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCGAAACCTTATT31591.139951717144744No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT31371.132012832125059No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA30861.1136090532157896No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATCCACTTACCAATCCACTC29631.069223468787552No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC29241.0551499907981106No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT28831.040354795988698No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT27700.9995777956603167No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC26110.9422013084725946No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC25890.9342624234529098No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT24560.8862682549248152No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC19520.7043956162920355No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA18870.6809398196429667No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC15720.5672694204974794No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC15380.5550002345579664No Hit
TAATCTAAACCTCCTAACCTCAAACAATCTATCTACTTCTACCTCCACCT15360.5542785177379952No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAACTTAACTATATC14780.5333487299588261No Hit
AACCTCCTCTCCAAAACTAAATCTTACACGCCCCCAAAATTAATTTATAC12890.46514649047153367No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA11930.43050408311290894No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACACTACCTT10460.3774578968450149No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC10060.363023560445588No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCGCCCCCTAA10020.3615801268056453No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACAAAAAAAATAATCACA10000.3608584099856739No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAC9600.346424073586247No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAACAACAT8670.3128642414575793No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCCAAAAATTTCTAAAAT8410.3034819227979518No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA8010.2890475863985248No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT7840.28291299342876836No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCACATCG7500.27064380748925543No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT7080.25548775426985715No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA7050.2544051790399001No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA6540.23600140013063076No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAACAACACAA6480.23383624967071667No Hit
AAAAAAAATCCCAAAACAAACATACAAAATCAAAAAAATACCTAAAATAA6470.23347539126073102No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA6360.2295059487508886No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC6180.22301049737114648No Hit
CCCAAAAATTAATAAATAAACCCCAAAAACTAAAAAATAAACGCCAAAAA6060.21868019645131842No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA6020.2172367628113757No Hit
ATACAAAACAAACCCCTATCTCCTCTACCACGACCTTAAAAAACAATACC5820.21001959461166222No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCACAC5810.20965873620167655No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA5690.20532843528184846No Hit
TAATCTAAACCTCCTAACCTCAAACAATCTATCTACTTCTACCTCCGCCT5410.1952243998022496No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAACAAAAACACCTAACGCTAA5330.1923375325223642No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCAAAAAATAAACCCCTA5270.19017238206245016No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCGAACATCAAAACCTTCATC5230.18872894842250745No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCAAACATTAAAACCTTCATC5220.18836809001252178No Hit
ACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC4550.16419057654348163No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCGCCAAAAAAACCC4430.15986027562365354No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCGCAA4210.15192139060396873No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA4040.14578679763421226No Hit
CCTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC3990.14398250558428388No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA3890.14037392148442715No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT3480.12557872667501452No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACACT3470.12521786826502887No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAAACAAAAACACCTAACGCT3440.12413529303507184No Hit
AATCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC3350.12088756734520076No Hit
CCATAAAAACAAAAACCTAAAAAAACTAAAACACCTATTAACCTATCCCG3060.11042267345561622No Hit
CCCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA3010.10861838140568784No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCACAA2960.10681408935575949No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC2880.10392722207587408No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)