FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888269

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888269
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences262428
Sequences flagged as poor quality0
Sequence length301
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT76892.9299464996113223No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC75712.884981785480208No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT70402.6826405718901944No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT63032.40180163702044No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC57712.19907936653101No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA53862.052372460255765No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA53232.0283658755925438No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA51851.9757800234731049No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA51591.9658725440882832No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT51171.9498681543128018No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA50501.9243373420519152No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT49791.8972823021933636No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC47361.8046854756352217No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC44341.6896062920115231No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT44221.6850336092185285No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA43251.648071089975155No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC41841.5943420671574682No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC41171.5688112548965811No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC40191.5314676787537915No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCACATCA36271.3820933741826331No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA35381.3481793101345891No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC33421.27349215784901No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC33291.268538418156599No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA32211.2273842730196474No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA31971.218238907433658No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA29381.119545170484857No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA28821.0982059841175484No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT25920.9876994832868444No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG25410.9682655814166172No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC25110.9568338744341305No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC24850.9469263950493088No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA24410.9301598914749951No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCGAAACCTTATT23820.907677534409438No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT23770.9057722499123569No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCGATTCACCCA23480.8947215998292865No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA22940.8741445272608107No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACGCCAAACCCCCACAAAAC22570.8600454219824104No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT20730.7899309524898257No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACACT18490.7045742070205924No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT18390.7007636380264302No Hit
AAACTACAAAATCAAAACCAACCCGAAACCGCGCCAAACCCCCGCAAAAC17180.6546557531970674No Hit
ACTCCCACACACACTAACACAATAAAACGACGCTACGAACATCAATACAC16170.6161690063560291No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACGCCAACAT14840.5654884387336717No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC14520.5532946179523527No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC14460.5510082765558554No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC13040.4968981968387519No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCGAACCCTAAATCCCGCC13000.495373969241087No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC12960.4938497416434222No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC12500.47632112427027606No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT11670.44469340161872967No Hit
CCCAAAAATTAATAAATAAACCCCAAAAACTAAAAAATAAACGCCAAAAA11410.43478592223390794No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA10300.3924886063987074No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCGAACCCTAAATCCCACC10130.3860106391086317No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA9940.3787705580197235No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA9380.3574313716524151No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC9270.3532397457588367No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA9150.3486670629658421No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACGAAAAAAATAATCACA8750.3334247869891932No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC8670.33037633179386344No Hit
AATCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC8100.3086560885271389No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA7110.270931455484933No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCAAACATTAAAACCTTCATC6330.2412090173304678No Hit
AATATTTAAAAAACCCTACCTTAAAAACGCATTCACTTACCGATCCACTC6290.23968478973280288No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA6240.23777950523572178No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCACAA5510.20996235157833767No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACGAA5430.2069138963830079No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA4540.17299983233496427No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTGACGCTA4530.17261877543554804No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCAACCTTAAAAAACAATACC4510.1718566616367156No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAACAACACAA4430.1688082064413858No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACGCCTCCAT4210.16042495465422896No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAGTACACGACC4130.1573764994588992No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAACAAAAACACCTAACGCTAA4110.15661438566006677No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT4000.15242275976648834No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACAAAAAAAATAATCACA3610.13756154068925572No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAAACAAAAACACCTAACGCT3460.1318456871980124No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAAACAAATCCCACATC3420.13032145960034752No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCACAC3340.12727300440501774No Hit
CCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA3130.11927080951727712No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA3110.11850869571844469No Hit
AAACACAAACCTATCTCTTATCCCCTGCGCCCGAAAAAAAAAACGACACA3100.11812763881902846No Hit
CCTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT2820.10745804563537427No Hit
CCTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC2790.10631487493712562No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)