FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888271

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888271
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences331810
Sequences flagged as poor quality0
Sequence length301
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC113103.4085771977939183No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT107673.2449293270245017No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT98392.9652511979747445No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT91652.7621229016605886No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC86972.6210783279587715No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA85302.570748319821585No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC82522.4869654320243515No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA78342.3609897230342667No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC75222.266960007233055No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT73662.2199451493324496No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC70422.1222989060004216No Hit
TCCAACCAATAACACTATCTCTAACCTCAATTTCCTCATCTATCCTATAA69322.0891474036346103No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT65631.9779391820620236No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA63511.914047195684277No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCACCT61971.8676350923721405No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA59531.7940990325788855No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC59221.7847563364576113No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA58331.757933757270727No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC53041.598505168620596No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC51971.5662577981374886No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC50631.525873240710045No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCACATCA50531.5228594677676983No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA49281.485187305988367No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA49031.4776528736325005No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT43551.312498116391911No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA42521.2814562550857418No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT40011.2058105542328441No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT40011.2058105542328441No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA36551.1015340104276543No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC35271.0629577167656188No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACGAA33441.0078056719206774No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT29820.8987070914077333No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC24080.7257165245170428No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC23510.7085380187456678No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA18920.5702058406919622No Hit
ATACAAAACAAACCCCTATCTCCTCTACCACGACCTTAAAAAACAATACC17600.5304240378529882No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG17290.5210813417317139No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC17230.5192730779663061No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC16760.5051083451372774No Hit
CCATAAAAACAAAAACCTAAAAAAAAACTAAAACACCTATTAACCTATCC15090.45477833700009035No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA14970.4511618094692746No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT14150.4264488713420331No Hit
AAACACAAACCTATCTCTTATCCCCTACGCCCAAAAAAAAAAACAACACA13610.4101744974533619No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACCAATTCCGCACCAACAT13400.4038455742744341No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAACTTAACTATATC12520.37732437238178473No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA12190.3673789216720412No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAACAACAT10960.330309514481179No Hit
AAACACAAACCTATCTCTTATCCCCTACGCCCAAAAAAAAAACAACACAA8960.2700340556342485No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC8860.267020282691902No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA7470.22512883879328532No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC6470.1949911093698201No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAAATCCTATAACTCTAA6220.18745667701395377No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAACAAAAACACCTAACGCTAA6120.18444290407160724No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA6120.18444290407160724No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA5570.16786715288870135No Hit
CCTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC5320.16033272053283507No Hit
CCTAAAAAAACAACACTCCAAACACGTATACCCAAACCCTAAATCCCACC5110.15400379735390735No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCACCAAAAAAACCC4610.13893493264217474No Hit
CCTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT4530.13652391428829752No Hit
CATAATCACCCACAACAAATTTCCCACCACTAAAATAAACCACCCCCTAA4450.1341128959344203No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAAACAAAAACACCTAACGCT4420.13320876405171633No Hit
CCCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA4280.1289894819324312No Hit
CCATAAAAACAAAAACCTAAAAAAACTAAAACACCTATTAACCTATCCCG4140.12477019981314609No Hit
CCCAACCAATAACACTATCTCTAACCTCAATTTCCTCATCTATCCTATAA4010.12085229498809559No Hit
CCTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA3900.11753714475151442No Hit
AAACACAAACCTATCTCTTATCCCCTACGCCCAAAAAAAAAAAACAACAC3850.11603025828034116No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCACAC3780.11392061722069859No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCGAACCCTAAATCCCGCC3650.11000271239564811No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAGAATCCTATAACTCTAAA3430.10337241192248577No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAACAACATT3400.1024682800397818No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)