FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888273
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences248518
Sequences flagged as poor quality0
Sequence length301
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT63952.5732542512011203No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA60182.421554977909045No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT58472.3527470847182097No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC56922.290377356972131No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC56102.2573817590677536No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC55322.225995702524566No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC54782.204266894148512No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC54662.1994382700649453No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA51232.061420098342977No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA50172.0187672522714655No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG47421.9081112836897125No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA46401.86706797897939No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA45871.8457415559436339No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA44021.7713002679886367No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA39471.5882149381533734No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA39461.5878125528130758No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT39421.586203011451887No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC37421.5057259433924304No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC35501.428467958055352No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT35361.42283456329119No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT35131.4135797004643527No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC35111.4127749297837582No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCACCT34531.3894365800465158No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC34411.3846079559629485No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACACACACTTCAAT33851.3620743769063006No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC33501.3479908899958957No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT33431.3451741926138148No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCGCCCCCTAA30581.2304943706290892No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA29721.195889231363523No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT28671.1536387706323084No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA28611.1512244585905247No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC26971.0852332627817702No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC26911.0828189507399868No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCGAAACCTTATT24800.9979156439372601No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCCAAAAATTTCTAAAAT24450.9838321570268551No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC24310.9781987622626933No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA24200.9737725235194231No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT23990.9653224313731803No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC23930.9629081193313965No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA21770.8759928858271836No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT18720.7532653570365124No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC16600.6679596648934886No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA16570.6667525088725967No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC16390.6595095727472456No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC13190.5307462638521153No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCAAACATTAAAACCTTCATC11300.454695434535929No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT10410.41888313924947085No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAACTTAACTATATC10070.40520203767936325No Hit
ATACAAAACAAACCCCTATCTCCTCTACCACGACCTTAAAAAACAATACC9170.3689873570526078No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA8420.33880845653031166No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCGCAAAAC8190.3295535937034742No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT8130.3271392816616905No Hit
AACCCTAACTCCCTTACAACTTCACCCCACACTATCAACTAAAAACAAAA7250.29172937171552965No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA7010.2820721235483949No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCACAA6970.28046258218720577No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCGAACATTAAAACCTTCATC6670.2683910219782873No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACGAA6230.25068606700520685No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA5810.233785882712721No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA5580.2245310198858835No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA5540.2229214785246944No Hit
TAATCTAAACCTCCTAACCTCAAACAATCTATCTACTTCTACCTCCGCCT5490.22090955182320798No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAACAAAAACACCTAACGCTAA4520.18187817381437157No Hit
ACTCCCACACACACTAACACAATAAAACAACACTACGAACATCAATACAC4290.1726233109875341No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAACCAATACC4290.1726233109875341No Hit
AAACACAAACCTATCTCTTATCCCCTACGCCCAAAAAAAAAAACGACACA3920.15773505339653465No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA3900.1569302827159401No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAACAACAT3820.15371119999356184No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC3780.1521016586323727No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCACCAAAAAAACC3600.14485872250702161No Hit
CCATAAAAACAAAAACCTAAAAAAACTAAAACACCTATTAACCTATCCCG3550.14284679580553522No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCGCAA3490.1404324837637515No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCCAAAACCTCAACGAA3420.13761578638167055No Hit
CCTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC3250.13077523559661675No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCGCCAAAAAAACCC2920.11749651936680643No Hit
ACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC2780.11186312460264448No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA2740.11025358324145534No Hit
CCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA2640.10622972983848253No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAAACAAAAACACCTAACGCT2640.10622972983848253No Hit
CCCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA2610.10502257381759068No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCACCAAAAAAACCC2570.10341303245640154No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCGAAAAAAAAAACAACACA2540.1022058764355097No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)