FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888275

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888275
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences260340
Sequences flagged as poor quality0
Sequence length301
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC86383.3179688100176694No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT71142.732580471690866No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT70392.7037719904739954No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT65602.5197818237689176No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA63442.436813397864331No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC61492.3619113467004684No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT61292.3542290850426366No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA59652.2912345394484137No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC57302.200967964968887No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA55492.1314434969655065No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA52912.032342321579473No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA52602.0204348160098333No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC49951.9186448490435584No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA49821.9136513789659675No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT45131.7335023430898056No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC44781.7200583851885993No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC44331.7027732964584774No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC42841.6455404471076285No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC42691.6397787508642545No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA41041.57640009218714No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT39201.5057232849350848No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA39171.50457094568641No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC38221.4680802028117077No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG37891.4554044710762848No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC37701.4481063225013444No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA37391.4361988169317048No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT34701.3328723976338634No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA34131.310977951909042No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA32971.266420834293616No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT32781.2591226857186757No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA29591.1365906122762541No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT28591.0981793039870937No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA27431.0536221863716677No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC26041.0002304678497351No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC23030.8846124298993624No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCACATCA16900.6491511100868095No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCGAACCCTAAATCCCGCC16890.6487669970039179No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC14970.5750172850887301No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT13760.5285396020588461No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC12900.49550587693016823No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCACAC12240.4701544134593224No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA12190.4682338480448644No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCGAACCCTAAATCCCACC11700.44941230698317586No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACGAAAAAAATAATCACA11100.42636552200967964No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA10310.396020588461243No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT10160.39025889221786897No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACAAACATCAATACAC8920.34262886993931013No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA8620.331105477452562No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC8620.331105477452562No Hit
ACTCCCACACACACTAACACAATAAAACAACACTACGAACATCAATACAC8160.31343627563954823No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAACTTAACTATATC8010.30767457939617426No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCACCACCCGCAAA7950.30536990089882465No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA7400.28424368133978645No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCAACCTTAAAAAACAATACC6340.2435276945532765No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC6050.23238841514942No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA5270.20242759468387492No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA5230.2008911423523085No Hit
AAACACAAACCTATCTCTTATCCCCTACGCCCAAAAAAAAAAACGACACA4870.18706307136821082No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAACAAAAACACCTAACGCTAA4700.18053314895905356No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCCCAAAACGACCATCAAACA4190.1609433817315818No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC3940.1513405546592917No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAACAACACAA3870.14865176307905048No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCGCAAAAC3860.14826764999615888No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAAACAAAAACACCTAACGCT3460.13290312668049473No Hit
CACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC3460.13290312668049473No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC3390.13021433510025351No Hit
AAAAAAATCCTTCCAACCAATAAAACTAACAATACCTACCCAACATGTAG3370.1294461089344703No Hit
CCATAAAAACAAAAACCTAAAAAAACTAAAACACCTATTAACCTATCCCG3130.12022739494507184No Hit
AAACACAAACCTATCTCTTATCCCCTACGCCCAAAAAAAAAACGACACAA3070.11792271644772222No Hit
CCTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA3000.11523392486748099No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCGCCAAAAAAACCC2650.10178996696627488No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)