FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888277

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888277
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences269335
Sequences flagged as poor quality0
Sequence length301
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT96293.5751016392225297No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC86173.1993613900904077No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT78472.9134720700985763No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT75262.7942896392967866No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA68502.543301093433828No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC67202.495034065383259No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA66372.4642174243971264No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT65132.4181781053335065No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC63622.362114095828615No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG61672.2897135537527613No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA55022.042809141032543No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA54632.0283290326173726No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC54372.0186756270072586No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA53251.9770917259175378No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT47121.7494941244175468No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT46921.7420684277943823No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT46041.7093953626524587No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC45081.6737520188612693No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA43441.6128613065513209No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC43401.6113761672266878No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC43271.6065494644216312No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCGAAACCTTATT42281.569792266136967No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA41971.5582824363710621No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC39511.4669463679061392No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA36961.3722687359607924No Hit
AAACTACAAAATCAAAACCAACCCGAAACCGCGCCAAACCCCCGCAAAAC32821.218556815861288No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA31811.1810570479143074No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC31601.1732600664599848No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA30151.1194237659420423No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC29711.1030872333710806No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC26760.9935582081794049No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA26130.9701672638164368No Hit
AATATTTAAAAAACCCTACCTTAAAAACGCATTCACTTACCAATCCACTC24060.8933113037666847No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC23020.8546976813262294No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC18770.6969016280839846No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCACAA18240.6772235320325988No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT16530.6137338259045426No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC14190.5268531754135185No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA12840.47672972320715834No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA12630.46893274175283567No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAAGTCCC10710.39764605417045684No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA9700.36014628622347633No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCGTCTAAAAATTTCTAAAAT9420.3497503109510461No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA8840.32821579074386914No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC7950.2951714407707873No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT7930.2944288711084709No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAACAACACAA7730.28700317448530643No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC6730.24987469136948412No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA6580.24430541890211074No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACGAACATTAAAACCTTCATC6330.23502329812315514No Hit
CCACACTCCTAAAAACCTAACTTCCACACAAATTTCCCACACTACACCCT6220.23093916498041472No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA5880.21831548072103515No Hit
ACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC5740.21311749308482003No Hit
ATACAAAACAAACCCCTATCTCCTCTACCACGACCTTAAAAAACAATACC5570.2068056509551302No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC4730.1756177251378395No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCGAT4360.16188018638498525No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCGCCAAAAAAACCC4310.16002376222919412No Hit
CCTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC4020.14925650212560565No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCCCATCTATCCTATAA4000.1485139324632892No Hit
CCTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT3300.12252399428221361No Hit
CCTAAAAAAACAACACTCCAAACACTCCAACCCCACTCTATCTTACAACC3230.11992500046410604No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAAATCCTATAACTCTAA3140.11658343698368204No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAAACAACAC2990.11101416451630869No Hit
CCATAAAAACAAAAACCTAAAAAAACTAAAACACCTATTAACCTATCCCA2990.11101416451630869No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT2930.10878645552935935No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAACAACACAAA2720.10098947407503665No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATGTAAAACCACCAAACGCT2720.10098947407503665No Hit
CACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA2710.10061818924387844No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)