FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888279

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888279
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences271894
Sequences flagged as poor quality0
Sequence length301
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT106133.9033593974122267No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT88273.2464857628340456No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC75562.7790241785401664No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT73222.692961227537202No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA67632.4873664001412314No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT66952.4623566536959256No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA66192.4344045841394073No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC60142.211891398853965No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA59622.192766298631084No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT57952.1313453036845242No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA56532.079119068460503No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT49651.8260792808962316No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA45751.6826410292246246No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC45681.6800664965023133No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT45001.6550567500570073No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA44591.6399773441120438No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA43211.5892222704436287No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC43091.58480878577681No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA42301.5557533450535872No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT42021.5454552141643434No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT41131.5127218695521047No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA40981.5072050137185815No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA40461.4880799134957006No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA32911.2103981698750248No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC32191.1839172618741127No Hit
AATATTTAAAAAACCCTACCTTAAAAACGCATTCGCTTACCGATCCACTC30821.1335299785945994No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCACATCG30391.1177149918718325No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC29931.1007966339823607No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC29581.0879239703708063No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC28831.0603396912031895No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC27381.0070100848124637No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC26270.9661853516443908No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG25730.9463246706437066No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC24090.8860070468638512No Hit
AATATTTAAAAAACCCTACCTTAAAAACGCATTCGCTTACCAATCCACTC22430.8249538423061928No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCGCC21240.7811867860269076No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT17250.6344384208551862No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA14250.5241013041847191No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCGATCCACTC14150.5204234002957036No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC14100.5185844483511958No Hit
ACTCCCACACACACTAACACAATAAAACGACACTACAAACATCAATACAC12500.4597379861269465No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA11340.4170743010143659No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACAAACATCAATACAC10750.395374668069174No Hit
ACTCCCACACACACTAACACAATAAAACGACGCTACGAACATCAATACAC10600.38985781223565064No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA10080.3707327120127697No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCGAACATTAAAACCTTCATC8960.3295401884557953No Hit
AAACTACAAAATCAAAACCAACCCGAAACCACACCAAACCCCCACAAAAC8430.31004729784401275No Hit
ACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC7940.2920255687878364No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA7860.28908324567662397No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA7650.2813596475096913No Hit
CTCATTCAAAAAACCCAAAAAACCGAACCCAACTCGTTCCGCGCCAACGT7540.27731395323177416No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACACCTCCAT7430.273268258953857No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA7310.2688547742870383No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACGAACATTAAAACCTTCATC7120.2618667568979087No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC6990.2570854818421885No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA6290.2313401546190795No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACGAAAAAAATAATCACA5820.21405400634070632No Hit
AATACCTAAAAACCCAAAACCCTTCCCACACGCACACACCATAAAAAAAC5800.2133184255629032No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAACAACACAA5700.20964052167388764No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACGCCTCCAT5590.2055948273959705No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC5330.19603227728453No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACGACACA5320.19566448689562846No Hit
CCACACTCCTAAAAACCTAACTTCCACGCACAAATTTCCCACACTACACC5190.1908832118399082No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCGCCAAAAAAACCC4980.18315961367297548No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACT4970.18279182328407392No Hit
CCTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT3940.14490941322721354No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC3820.14049592856039486No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAACCCAATTCACCCAT3760.13828918622698552No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAATACAACAT3670.1349790727268715No Hit
TACTATCCACACTCTCTTACCTCCCGACCCCCGCACTCCCTTCTAACACC3430.12615210339323413No Hit
CAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT3400.12504873222652946No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA3330.12247419950421855No Hit
CACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC3240.11916408600410454No Hit
AATCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC3120.11475060133728586No Hit
CCCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA3030.11144048783717182No Hit
CCTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA2890.10629142239255004No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAACGACACAA2850.10482026083694382No Hit
AAAAAAATCCTTCCAACCAATAAAAACGGTAACTAACCAAATAAATAACT2790.10261351850353448No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA2780.10224572811463291No Hit
CCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA2720.10003898578122357No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)