FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888281

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888281
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences265945
Sequences flagged as poor quality0
Sequence length301
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC83673.1461392393164No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT76472.8754065690274304No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT75682.845701178815169No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT72322.719359266013649No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC62272.3414615804019627No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT62142.336573351632856No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC59982.2553535505461655No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT53161.9989095489668915No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA51191.9248340822350485No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA51141.9229539942469307No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA51121.9222019590516837No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA50771.9090413431348585No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA48971.8413581755626165No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC47701.7936039406644229No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC45761.7206565267254508No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT41131.5465603790257383No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA41041.5431762206471265No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT40771.5330237455112898No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA39771.495421985748933No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC39131.4713568595010247No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC38761.4574442083889525No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC37451.408185903100265No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA36591.375848389704638No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC35291.3269661020135741No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC34851.3104213277181371No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA34171.2848521310797345No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA31431.1818233093308765No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG31311.1773110981593937No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA31011.1660305702306868No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT29581.1122600537705165No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA26510.9968226513000809No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC24910.9366598356803097No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC24450.9193630261896256No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA22450.844159506664912No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT21790.8193423452217564No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC19970.7509071424542668No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC17650.6636710598055989No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCGATCCACTC14620.5497377277256575No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCACAA14340.5392092349921976No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACCC14330.538833217394574No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC14080.5294327774539849No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC13330.5012314576322172No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA12710.47791836657955594No Hit
CCAACATATACAAACACAAAATACTCAACGACTACCTACACAAACAAACC11750.4418206772076933No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA11570.435052360450469No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAGCCACCCCCTAA11460.4309161668766099No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA10980.4128673221906785No Hit
ATACAAAACAAACCCCTATCTCCTCTACCACGACCTTAAAAAACAATACC10840.40760307582394856No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT10420.39181033672375865No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT9840.37000131606159165No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA9810.36887326326872094No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA9500.35721671774239033No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAACTTAACTATATC9180.34518415461843616No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCAAAAAATAAACCCCTA7950.2989339901107372No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCCAAAACCTCAACAAA7720.2902855853653951No Hit
CCCAAAAATTAATAAATAAACCCCAAAAACTAAAAAATAAACGCCAAAAA7210.2711086878865931No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA7000.2632123183364981No Hit
AAACTACAAAATCAAAACCAACCCGAAACCACACCAAACCCCCACAAAAC6400.24065126247908403No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA6330.23801913929571905No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCGCAA6170.2320028577337419No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAACAACACAA6160.23162684013611837No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAGGGCGACCATCAAACA5920.2226024177931527No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC5740.21583410103592848No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC5130.1928970275808908No Hit
ACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC5050.18988888679990223No Hit
TAAAACTAAACCCCACATACCTTCCTCCTAATATAAAACCACCAAACGCT4780.17973641166406587No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTC4760.17898437646881876No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAACAAAAACACCTAACGCTAA4610.1733441125044652No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA4520.16995995412585307No Hit
AATATTTAAAAAACCCTACCTTAAAAACGCATTCGCTTACCGATCCACTC4500.16920791893060594No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAGAACCTTCATC4010.15078305664705108No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCAAACATTAAAACCTTCATC3930.14777491586606253No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAACAACAT3550.13348624715636692No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCGCCAAAAAAACCC3160.11882156084904773No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA2890.10866908571321138No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAAACAACAC2710.10190076895598714No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)