FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888287

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888287
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences302164
Sequences flagged as poor quality0
Sequence length301
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC102973.407752081651024No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT95283.153254524033306No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT91133.0159118889080103No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT85662.834884367429608No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA71792.3758621146132564No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT71202.3563362941978525No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA65792.177294449371864No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA64622.138573754649793No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC61502.0353185687242688No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC61312.0290305926582914No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA60311.9959359817847262No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC59831.9800505685654148No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT59671.9747554308256445No Hit
ACTCCCACACACACTAACACAATAAAACGACGCTACGAACATCAATACAC59051.9542367720840337No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA55791.8463483406362107No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA48601.6083980884552758No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT45121.4932288426152684No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT43921.4535153095669902No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA40881.3529076925113515No Hit
AAACTACAAAATCAAAACCAACCCGAAACCACACCAAACCCCCACAAAAC39861.319151189420315No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC39781.3165036205504295No Hit
ATACAAAACAAACCCCTATCTCCTCTGCCGCGACCTTAAAAAACAATACC39721.3145179438980157No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC39121.2946611773738765No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC37381.2370765544538727No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG36631.2122555962986987No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT33721.1159502786566236No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC33631.1129717636780028No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA32411.0725963384122528No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA32361.0709416078685747No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA31871.0547252485405276No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT30120.9968096795117882No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA29720.9835718351623621No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA27490.9097708529143114No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC26430.8746905653883321No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACGAA26100.8637693438000557No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCGATCCACTC23920.7916230920956832No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC23440.7757376788763717No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA22070.7303980619795872No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC20730.6860512834090097No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC19100.6321070676850982No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC18020.5963648879416475No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACGAA17020.5632702770680822No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC15370.5086641691266994No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACAACTTAACTATATC14490.4795409115579619No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC12200.40375425265749726No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA10810.35775274354324144No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT10610.35113382136852833No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACGAAAAAAATAATCACA10230.33855786923657355No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAACAACAT10160.3362412464754239No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA9300.3077798811241578No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAACGCCTCCAT8700.2879231146000185No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAACAACACAA7730.25582134205266016No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA7430.24589295879059053No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC6920.22901470724507222No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA6800.22504335394024438No Hit
ACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC6060.20055334189380603No Hit
CCTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT5240.17341576097748243No Hit
AAAAAAATCCTTCCAACCAATAAAACTAACAATACCTACCCAACATGTAG5050.167127784911505No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAATACCTAAAATAAA4760.15753034775817107No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAACAAAAACACCTAACGCTAA4750.1571994016494354No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA4650.1538899405620789No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC4560.150911425583458No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCGAACA4550.15058047947472233No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACGCC4000.1323784434942614No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAACAACATT3710.12278100634092744No Hit
CCTCCCACACACACTAACACAATAAAACGACGCTACGAACATCAATACAC3550.11748586860115698No Hit
CTAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA3340.11053600031770826No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAACTCAAAACCTTATT3120.10325518592552388No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC3090.10226234759931692No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)