FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences300577
Sequences flagged as poor quality0
Sequence length301
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT82572.7470498408061825No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT82202.7347401830479376No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT73362.4406391706617603No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC65512.1794748101152117No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC63132.100293768318933No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC62892.0923091254487205No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT62392.0756744528024433No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA61532.047062815850847No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA61162.034753158092602No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA61052.031093530110421No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT59021.9635567591665362No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA56031.8640814167417998No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC55791.856096773871587No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA52111.7336655831949883No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC51221.7040558658846152No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT48381.609570925253762No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCATCTATCCTATAA47121.5676515501851438No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA46041.5317206572691855No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC41611.3843374576231713No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA39471.3131410586971057No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA38821.2915159842569457No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC38381.2768774723282221No Hit
ACTCCCACACACACTAACACAATAAAACGACGCTACGAACATCAATACAC37101.2342927103537529No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA37081.2336273234479018No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC36881.226973454389391No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA34221.1384769959111976No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA32031.0656171297205042No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA31981.0639536624558765No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC31911.0616248082853976No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT31221.0386689600335355No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC30091.0010745998529496No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC26720.8889569062170425No Hit
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACAACC25470.8473702246013501No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC25300.8417144359016159No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAACAACAT24520.8157643465734239No Hit
AACCCCAAATACAAAAAAATACACCACCAAAACCGAAAAATAAACCCCTA22910.7622007006524119No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC19730.6564041826220902No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC18480.6148175010063978No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA18130.6031732301540038No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCGCTTACCAATCCACTC16910.5625846288970879No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC15830.5266537359811296No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCGCAA14350.4774151049481497No Hit
CCCAAAAATTAATAAATAAACCCCAAAAACTAAAAAATAAACGCCAAAAA13180.4384899709558615No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTCAACTATATC12630.4201918310449569No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACAACCATCAAACA11940.3972359827930946No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC11890.3955725155284669No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACACACACTTCAAT11820.39324366135798816No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACGAACATTAAAACCTTCATC11760.3912475006404349No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCGAACATTAAAACCTTCATC11020.36662818512394496No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACGCCAAACCCCCACAAAAC10550.3509915928364446No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT10500.34932812557181686No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACACTACCTT10060.33468961364309313No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCCCTCTAAAAATTTCTAAA10050.33435692019016755No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAACAACATT9160.3047472028797945No Hit
AAAAAAATCCTTCCAACCAATAAAACTAACAATACCTACCCAACATGTAG8900.2960971731037305No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA8400.2794625004574535No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT7580.2521816373175592No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAACAACACAA7220.2402046730122398No Hit
AAAACCCCACTCTATCTTACAACCTAAATATCCCCTGTAGAACCATGTCG7180.23887389920053764No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAGCCCCCACCACCCGCAA7070.2352142712183567No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCGTTAACTCCC6650.22124114619548405No Hit
AAAACCCCACTCTATCTTACAACCTAAATTCAAAACCCAAGCGCCTCCAT6150.20460647354920702No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCGCCAAAAAAACC6030.20061415211410052No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA5930.19728721758484513No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAACAAAAACACCTAACGCTAA5180.17233520861542967No Hit
ACTCCCACACACACTAACACAATAAAACAACACTACGAACATCAATACAC4740.1576966966867059No Hit
AAACTACAAAATCAAAACCAACCCCATACAACTCCCTAATACCTTGTAGA4600.15303898834574833No Hit
ACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC4510.1500447472694185No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACGAAACGCACACTTCAAT4500.14971205381649294No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACGAAAAAAATAATCACA4340.1443889585696843No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCGAACCCTAAATCCCACC4160.13840047641702458No Hit
CATAATCACCCACAACAAATTTCCCGCCACCAAAATAAACCACCCCCTAA4030.13407546152899258No Hit
CCTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT3780.12575812520585408No Hit
TCCAACCAATAACACTATCTCTAACCTCGATTTCCTCACCTATCCTATAA3730.12409465794122637No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCGATCCACTC3680.12243119067659868No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCACATCA3670.12209849722367312No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAACCTACCGCCAAAAAAACCC3660.1217658037707476No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCACAC3520.11710809542979003No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAAACAACA3440.11444654780638573No Hit
AAAAAAATCCTTCCAACCAACAAAACTAACAATACCTACCCAACATGTAG3310.11012153291835369No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT3240.10779267874787492No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAATAATCACAC3230.10745998529494939No Hit
CCATAAAAACAAAAACCTAAAAAAACTAAAACACCTATTAACCTATCCCG3160.1051311311244706No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)