FastQCFastQC Report
Sun 4 Sep 2022
EGAF00000888291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00000888291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences279531
Sequences flagged as poor quality0
Sequence length301
%GC32

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACCATAAATACACAAAAAACAACCTATACCACTACTAAATACACGACC89093.1871241472323284No Hit
CCAACTAAAACTCAACATAATATCAAACAAAACAAAACGCACACTTCAAT86373.0898183028000474No Hit
AAACTACAAAATCAAAACCAACCCAAAACCACACCAAACCCCCACAAAAC84463.021489566452379No Hit
CTCATTCAAAAAACCCAAAAAACCAAACCCAACTCATTCCACACCAACAT79572.846553691719344No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT65372.338559945050817No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCGCCCCCTAA63832.2834676654825405No Hit
TAAAACTAAACCCCACATACCTTCCTCCAAATATAAAACCACCAAACGCT63772.2813212130318283No Hit
AACCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC62702.2430428109941296No Hit
ACTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA55992.0029978785894946No Hit
AAAAACCACAAAAACCTTCTCAATATTCATAAAAAAACCCAATTCACCCA55982.002640136514376No Hit
ATAATACTCCTTTATCCCAATCCCCAAAACCCTTCACCAAAAATACTAAA55351.9801023857818991No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC54181.9382465629930135No Hit
TAATCTAAACCTCCTAACCTCAAACGATCTATCTACTTCTACCTCCGCCT50311.7998003799220836No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCGAAAAATAAACCCCTA49641.7758316608891322No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAATACCTAAAATAA48641.740057453377264No Hit
AATAAAAAACTCCCTCTTCCAAAAAATTATTTAAAATTCAAAACCTTATT48461.7336180960251277No Hit
AACTTACACATCTCAAAAAAACCCAAAAAACCCCGCAACCACGCTACCTT44321.585512876925994No Hit
AAAACAAAAAAAACTCCTAATCCCTATAACCTCAAAACGACCATCAAACA40261.4402695944278094No Hit
TACTATCCACACTCTCTTACCTCCCAACCCCCACACTCCCTTCTAACACC37311.3347356822677985No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCGACCTTAAAAAACAATACC36631.3104092211597282No Hit
TCCAACCAATAACACTATCTCTAACCTCGACTTCCTCATCTATCCTATAA35831.2817898551502338No Hit
AATACCTTAACTCCCTAAAACCCAAATATAAATCAAAAATATCCCAAAAC35601.273561787422504No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAAATCCTATAACTCTAAA34631.238860806135992No Hit
CCTAAAAAAACAACACTCCAAACACATATACCCAAACCCTAAATCCCACC33211.1880614314691393No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCACAAACATTAAAACCTTCATC31151.114366563994691No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCA30831.1029188175908933No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCGATCCACTC29801.0660713838536693No Hit
TAATCTAAACCTCCTAACCTCAAACAATCTATCTACTTCTACCTCCGCCT28061.0038242627830187No Hit
CAAAACAAACAACACTAAAACCAAACTAATAAAAAACAAATCCCGCATCG28051.0034665207078999No Hit
AAAAAAATCCTTCCAACCAATAAAAACAATAACTAACCAAATAAATAACT25920.9272674587076211No Hit
CCAACATATACAAACACAAAATACTCAACAACTACCTACACAAACAAACC25880.9258364904071463No Hit
CCCCTAATAAAAACTTCCTTCCACTAACCGCGAACATTAAAACCTTCATC23980.857865496134597No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAACAACAT22980.8220912886227288No Hit
CCACACTCCTAAAAACCTAACTTCCACACACAAATTTCCCACACTACACC22690.811716768444287No Hit
AAAAATCCTAAAAACCCCAAAACTACGAACTAACGAAAAAAATAATCACA20800.7441035162468563No Hit
AAAAATCCTAAAAACCCCAAAACTACAAACTAACAAAAAAAATAATCACA19460.6961660781809531No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAAACAAAAACACCTAACGCTA18890.6757747798991883No Hit
TCTTTCCTCTTACCCAACACTTTATAAATAAATAATCACCAAAACCGCAC18580.6646847755705091No Hit
CACCCAAAATACCTCAACTACCTTACTCTAAACTAACCCCATTAACTCCC18270.6535947712418301No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCGCAA17010.6085192697768762No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAACAACAT16990.607803785626639No Hit
AATATTTAAAAAACCCTACCTTAAAAACACATTCACTTACCAATCCACTC16180.5788266775420258No Hit
AACCACTCACCTACTTAATCTCCCGATTCCAATATATCCAAAACTTACCC16010.5727450622650082No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAGCCCCCACCACCCGCAA13660.48867567461211814No Hit
CCCATAAACACCCTATAAACTAACATCACTAAACACGACTTAACTATATC13560.48509825386093136No Hit
ATACAAAACAAACCCCTATCTCCTCTACCGCAACCTTAAAAAACAATACC13150.47043082878106546No Hit
AACCCCAAATACAAAAAAATACACCACTAAAACCAAAAAATAAACCCCTA12590.4503972725744193No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAACAACATT12040.4307214584428919No Hit
ACCCCCAAAAAAACCTACTCATAAACGCTTAAAACCCCCACCACCCACAA11940.427144037691705No Hit
ACCCCCAAAAAAACCTACTCATAAACACTTAAAACCCCCACCACCCACAA9050.32375657798240626No Hit
CATAATCACCCACAACAAATTTCCCACCACCAAAATAAACCACCCCCTAA8700.3112356053532524No Hit
CAAAAAAACAACCTTCTCTCAATAAAAAAAACAAAAACACCTAACGCTAA7860.2811852710432832No Hit
AAAAAAATCCTTCCAACCAATAAAAACGATAACTAACCAAATAAATAACT6290.2250197652496503No Hit
AAAAAAAATCCCAAAACAAACATACGAAATCAAAAAAACACCTAAAATAA6210.22215782864870087No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAACAACATT5880.21035234016978438No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACAACACA5570.19926233584110528No Hit
CATAACACAAACACAAAAAACCAACCAAAAAAAATCCTATAACTCTAAAA5330.19067652603825694No Hit
ACCCTCCTCTCCAAAACTAAATCTTACACACCCCCAAAATTAATTTATAC5110.18280620038564596No Hit
ACTCCCTTTTCCCTTTAACTTTATTATATATCTCTCTAAAAATTTCTAAA5080.1817329741602899No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAACAACACAA4850.17350490643256025No Hit
CCATAAAAACAAAAACCTAAAAAAAACTAAAACACCTATTAACCTATCCC4020.14381231419770973No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCATTTTAAAAAAAAAAACAACA3980.142381345897235No Hit
CCTCCCTTTTCCCTTTAACTTTATTATATCCCTCTAAAAATTTCTAAAAT3750.13415327816950534No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAACGACACAA3570.1277139208173691No Hit
ACTCTAAAAAACCAACTCCTCCAAACGAAAACTCCTAAAACCTCAACAAA3240.11590843233845263No Hit
ACTCCCACACACACTAACACAATAAAACAACGCTACAAACATCAATACAC3220.11519294818821525No Hit
AATACCTAAAAACCCAAAACCCTTCCTCCGTTTTAAAAAAAAAAACAACA3170.11340423781262185No Hit
ACTCCCACACACACTAACACAATAAACAACGCTACGAACATCAATACACT3130.11197326951214713No Hit
AAACACAAACCTATCTCTTATCCCCTACACCCAAAAAAAAAAACGACACA3080.11018455913655373No Hit
CCTCCCACACACACTAACACAATAAAACAACGCTACGAACATCAATACAC3020.10803810668584164No Hit
TAAACACCAAACCCAAAAAAATAAAAAAAAAACCTACCACCAAAAAAACC2990.10696488046048559No Hit
CCTCTAAAAAACCAACTCCTCCAAACAAAAACTCCTAAAACCTCAACAAA2840.10159874933370538No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)